Reviewed, UniProtKB/Swiss-Prot A3Q338 (MHPB_MYCSJ)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	Mjls_3789

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.
Cofactor	Fe(2+) ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase		PRO_0000337656
Sites
	Active site	116	1	Proton donor By similarity
	Active site	180	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3Q338-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 2FE96B1CB6B9219F	FASTA	313	33,047
	10 20 30 40 50 60 MAQIALCCTS HSPLLNLPGP SRELLDDIGS ALAVARDFVT EFDPDLVVTF SPDHYNGFFY 70 80 90 100 110 120 KVMPPFCVGT SAQGVGDYGT HAGPLDVPED LANELATAVL EAGVDVAISA SMDVDHGTVQ 130 140 150 160 170 180 PLQNLFGDAM ARPVIPVFIN SVATPLGPLR RTRALGTAIG RYLATLDKRV LVIGSGGLSH 190 200 210 220 230 240 DPPVPTLATA PPAALDRIVH GAPMSTEQRM ARQSAVIDAA HAFAHGESPL QPLNPAWDAT 250 260 270 280 290 300 FLEILDEGWL SDLDGWSNAF IAREGGNSAH EIRTWVAAFA ALAAGGDYRT GLRFYRAAPE 310 LIAGFAIRTA VLA

References

[1]

"Complete sequence of Mycobacterium sp. JLS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000580 Genomic DNA. Translation: ABN99565.1.
RefSeq	YP_001072056.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4879499.
GenomeReviews	Gene locus Mjls_3789 in contig CP000580_GR.
KEGG	mjl:Mjls_3789.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 1 hit.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_MYCSJ

Accession

Primary (citable) accession number: A3Q338

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)