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A3Q1S8

- LIPA_MYCSJ

UniProt

A3Q1S8 - LIPA_MYCSJ

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Protein

Lipoyl synthase

Gene

lipA

Organism
Mycobacterium sp. (strain JLS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi92 – 921Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi99 – 991Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMSP164757:GHV3-3348-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:Mjls_3327
OrganismiMycobacterium sp. (strain JLS)
Taxonomic identifieri164757 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000002152: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Lipoyl synthasePRO_1000012236Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi164757.Mjls_3327.

Structurei

3D structure databases

ProteinModelPortaliA3Q1S8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A3Q1S8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVTPSGSNG AGSAAPEGRK LLRLEVRNAQ TPIERKPPWI KTRARMGPEY
60 70 80 90 100
KELKSLVKRE GLHTVCEEAG CPNIFECWED REATFLIGGE QCTRRCDFCQ
110 120 130 140 150
IDTGKPSELD RDEPRRVAES VQAMGLRYST VTGVARDDLP DGGAWLYAET
160 170 180 190 200
VREIKRLNPN TGVELLIPDF NGDPALLAQV FESRPEVLAH NVETVPRIFK
210 220 230 240 250
RIRPAFRYER SLAVLTAARD DNLVTKSNLI LGMGETPDEV RTALVDLHEA
260 270 280 290 300
GCDIITITQY LRPSPRHHPV ERWVKPEEFV EFAQFAEGLG FAGVLSGPLV
310 320
RSSYRAGRLY AQAARLKPAA TPPVS
Length:325
Mass (Da):36,147
Last modified:April 3, 2007 - v1
Checksum:i859A429D3DC1F029
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000580 Genomic DNA. Translation: ABN99105.1.
RefSeqiYP_001071596.1. NC_009077.1.

Genome annotation databases

EnsemblBacteriaiABN99105; ABN99105; Mjls_3327.
GeneIDi4879039.
KEGGimjl:Mjls_3327.
PATRICi18092836. VBIMycSp51234_3346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000580 Genomic DNA. Translation: ABN99105.1 .
RefSeqi YP_001071596.1. NC_009077.1.

3D structure databases

ProteinModelPortali A3Q1S8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 164757.Mjls_3327.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN99105 ; ABN99105 ; Mjls_3327 .
GeneIDi 4879039.
KEGGi mjl:Mjls_3327.
PATRICi 18092836. VBIMycSp51234_3346.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci MSP164757:GHV3-3348-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JLS.

Entry informationi

Entry nameiLIPA_MYCSJ
AccessioniPrimary (citable) accession number: A3Q1S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3