Reviewed, UniProtKB/Swiss-Prot A3Q0E7 (MASZ_MYCSJ)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Mjls_2844

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	Malate synthase G HAMAP MF_00641		PRO_1000056907
Sites
	Active site	342	1	Proton acceptor By similarity
	Active site	636	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3Q0E7-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: FB2D4F7132115B6C	FASTA	731	79,879
	10 20 30 40 50 60 MTDRVTVGNL RVARALYDFI TDEALAGTDL DPDSFWSGVD KVVTDLTPRN QELLARRDDL 70 80 90 100 110 120 QAQIDKWHRQ RAIGPHDADE YKQFLTEIGY LEPDPGDFTI TTAGVDDEIT TTAGPQLVVP 130 140 150 160 170 180 VLNARFALNA ANARWGSLYD ALYGTDVISD EDGAEKGTSY NRVRGDKVIA YAREFLDGAA 190 200 210 220 230 240 PLASGSYADA TGFRIEDGQV QVELGDDQWV GLAGPDQFVG YTGELGSPQW SILLRNNGLH 250 260 270 280 290 300 IEILIDPDSP VGSTDKAGVK DVVLESAVTT IMDFEDSVAA VDAEDKVLGY RNWLGLNRGD 310 320 330 340 350 360 LSEEVSKGDK TFTRVLNPDR TYTTPDGSGE LTLPGRSLLF VRNVGHLMTN DAITDAEGNE 370 380 390 400 410 420 VFEGIQDALF TGLIAMHGLK ESDANGPLRN SRTGSVYIVK PKMHGPAEVA YTVDLFSRVE 430 440 450 460 470 480 DVLGLPQNTL KVGIMDEERR TTLNLKACIK AAADRVVFIN TGFLDRTGDE IHTSMEAGPM 490 500 510 520 530 540 IRKGAMKSQP WIKAYEDQNV DVGLATGFSG RAQIGKGMWA MTDLMADMVE QKIGQPKAGA 550 560 570 580 590 600 TTAWVPSPTA ATLHAMHYHQ VDVYAVHKEL EGKQRASLDD LLTIPLAKEL AWAPEEIREE 610 620 630 640 650 660 VDNNCQSILG YVVRWIDAGV GCSKVPDIHD IALMEDRATL RISSQLLANW LRHGVITEED 670 680 690 700 710 720 VKTSLRRMAA VVDEQNAKDP DFKPMATDPD SSIAFQAAQE LILAGGTQPS GYTEPILHRR 730 RREYKASVAG A

References

[1]

"Complete sequence of Mycobacterium sp. JLS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000580 Genomic DNA. Translation: ABN98624.1.
RefSeq	YP_001071115.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3Q0E7.
Genome annotation databases
GeneID	4878558.
GenomeReviews	Gene locus Mjls_2844 in contig CP000580_GR.
KEGG	mjl:Mjls_2844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PKMHGPD.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_MYCSJ

Accession

Primary (citable) accession number: A3Q0E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)