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A3Q0E7

- MASZ_MYCSJ

UniProt

A3Q0E7 - MASZ_MYCSJ

Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium sp. (strain JLS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei277 – 2771Acetyl-CoAUniRule annotation
    Binding sitei314 – 3141Acetyl-CoAUniRule annotation
    Active sitei342 – 3421Proton acceptorUniRule annotation
    Binding sitei342 – 3421GlyoxylateUniRule annotation
    Metal bindingi437 – 4371MagnesiumUniRule annotation
    Binding sitei437 – 4371GlyoxylateUniRule annotation
    Metal bindingi465 – 4651MagnesiumUniRule annotation
    Binding sitei546 – 5461Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei636 – 6361Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMSP164757:GHV3-2858-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Mjls_2844
    OrganismiMycobacterium sp. (strain JLS)
    Taxonomic identifieri164757 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000002152: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 731731Malate synthase GPRO_1000056907Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei622 – 6221Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi164757.Mjls_2844.

    Structurei

    3D structure databases

    ProteinModelPortaliA3Q0E7.
    SMRiA3Q0E7. Positions 5-729.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni462 – 4654Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A3Q0E7-1 [UniParc]FASTAAdd to Basket

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    MTDRVTVGNL RVARALYDFI TDEALAGTDL DPDSFWSGVD KVVTDLTPRN    50
    QELLARRDDL QAQIDKWHRQ RAIGPHDADE YKQFLTEIGY LEPDPGDFTI 100
    TTAGVDDEIT TTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDVISD 150
    EDGAEKGTSY NRVRGDKVIA YAREFLDGAA PLASGSYADA TGFRIEDGQV 200
    QVELGDDQWV GLAGPDQFVG YTGELGSPQW SILLRNNGLH IEILIDPDSP 250
    VGSTDKAGVK DVVLESAVTT IMDFEDSVAA VDAEDKVLGY RNWLGLNRGD 300
    LSEEVSKGDK TFTRVLNPDR TYTTPDGSGE LTLPGRSLLF VRNVGHLMTN 350
    DAITDAEGNE VFEGIQDALF TGLIAMHGLK ESDANGPLRN SRTGSVYIVK 400
    PKMHGPAEVA YTVDLFSRVE DVLGLPQNTL KVGIMDEERR TTLNLKACIK 450
    AAADRVVFIN TGFLDRTGDE IHTSMEAGPM IRKGAMKSQP WIKAYEDQNV 500
    DVGLATGFSG RAQIGKGMWA MTDLMADMVE QKIGQPKAGA TTAWVPSPTA 550
    ATLHAMHYHQ VDVYAVHKEL EGKQRASLDD LLTIPLAKEL AWAPEEIREE 600
    VDNNCQSILG YVVRWIDAGV GCSKVPDIHD IALMEDRATL RISSQLLANW 650
    LRHGVITEED VKTSLRRMAA VVDEQNAKDP DFKPMATDPD SSIAFQAAQE 700
    LILAGGTQPS GYTEPILHRR RREYKASVAG A 731
    Length:731
    Mass (Da):79,879
    Last modified:April 3, 2007 - v1
    Checksum:iFB2D4F7132115B6C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000580 Genomic DNA. Translation: ABN98624.1.
    RefSeqiWP_011855814.1. NC_009077.1.
    YP_001071115.1. NC_009077.1.

    Genome annotation databases

    EnsemblBacteriaiABN98624; ABN98624; Mjls_2844.
    GeneIDi4878558.
    KEGGimjl:Mjls_2844.
    PATRICi18091857. VBIMycSp51234_2864.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000580 Genomic DNA. Translation: ABN98624.1 .
    RefSeqi WP_011855814.1. NC_009077.1.
    YP_001071115.1. NC_009077.1.

    3D structure databases

    ProteinModelPortali A3Q0E7.
    SMRi A3Q0E7. Positions 5-729.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 164757.Mjls_2844.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN98624 ; ABN98624 ; Mjls_2844 .
    GeneIDi 4878558.
    KEGGi mjl:Mjls_2844.
    PATRICi 18091857. VBIMycSp51234_2864.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci MSP164757:GHV3-2858-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JLS.

    Entry informationi

    Entry nameiMASZ_MYCSJ
    AccessioniPrimary (citable) accession number: A3Q0E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3