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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium sp. (strain JLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei277 – 2771Acetyl-CoAUniRule annotation
Binding sitei314 – 3141Acetyl-CoAUniRule annotation
Active sitei342 – 3421Proton acceptorUniRule annotation
Binding sitei342 – 3421GlyoxylateUniRule annotation
Metal bindingi437 – 4371MagnesiumUniRule annotation
Binding sitei437 – 4371GlyoxylateUniRule annotation
Metal bindingi465 – 4651MagnesiumUniRule annotation
Binding sitei546 – 5461Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei636 – 6361Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMSP164757:GHV3-2858-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Mjls_2844
OrganismiMycobacterium sp. (strain JLS)
Taxonomic identifieri164757 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000002152: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731Malate synthase GPRO_1000056907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei622 – 6221Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi164757.Mjls_2844.

Structurei

3D structure databases

ProteinModelPortaliA3Q0E7.
SMRiA3Q0E7. Positions 5-729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni462 – 4654Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A3Q0E7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVTVGNL RVARALYDFI TDEALAGTDL DPDSFWSGVD KVVTDLTPRN
60 70 80 90 100
QELLARRDDL QAQIDKWHRQ RAIGPHDADE YKQFLTEIGY LEPDPGDFTI
110 120 130 140 150
TTAGVDDEIT TTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDVISD
160 170 180 190 200
EDGAEKGTSY NRVRGDKVIA YAREFLDGAA PLASGSYADA TGFRIEDGQV
210 220 230 240 250
QVELGDDQWV GLAGPDQFVG YTGELGSPQW SILLRNNGLH IEILIDPDSP
260 270 280 290 300
VGSTDKAGVK DVVLESAVTT IMDFEDSVAA VDAEDKVLGY RNWLGLNRGD
310 320 330 340 350
LSEEVSKGDK TFTRVLNPDR TYTTPDGSGE LTLPGRSLLF VRNVGHLMTN
360 370 380 390 400
DAITDAEGNE VFEGIQDALF TGLIAMHGLK ESDANGPLRN SRTGSVYIVK
410 420 430 440 450
PKMHGPAEVA YTVDLFSRVE DVLGLPQNTL KVGIMDEERR TTLNLKACIK
460 470 480 490 500
AAADRVVFIN TGFLDRTGDE IHTSMEAGPM IRKGAMKSQP WIKAYEDQNV
510 520 530 540 550
DVGLATGFSG RAQIGKGMWA MTDLMADMVE QKIGQPKAGA TTAWVPSPTA
560 570 580 590 600
ATLHAMHYHQ VDVYAVHKEL EGKQRASLDD LLTIPLAKEL AWAPEEIREE
610 620 630 640 650
VDNNCQSILG YVVRWIDAGV GCSKVPDIHD IALMEDRATL RISSQLLANW
660 670 680 690 700
LRHGVITEED VKTSLRRMAA VVDEQNAKDP DFKPMATDPD SSIAFQAAQE
710 720 730
LILAGGTQPS GYTEPILHRR RREYKASVAG A
Length:731
Mass (Da):79,879
Last modified:April 3, 2007 - v1
Checksum:iFB2D4F7132115B6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000580 Genomic DNA. Translation: ABN98624.1.
RefSeqiWP_011855814.1. NC_009077.1.
YP_001071115.1. NC_009077.1.

Genome annotation databases

EnsemblBacteriaiABN98624; ABN98624; Mjls_2844.
GeneIDi4878558.
KEGGimjl:Mjls_2844.
PATRICi18091857. VBIMycSp51234_2864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000580 Genomic DNA. Translation: ABN98624.1.
RefSeqiWP_011855814.1. NC_009077.1.
YP_001071115.1. NC_009077.1.

3D structure databases

ProteinModelPortaliA3Q0E7.
SMRiA3Q0E7. Positions 5-729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi164757.Mjls_2844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN98624; ABN98624; Mjls_2844.
GeneIDi4878558.
KEGGimjl:Mjls_2844.
PATRICi18091857. VBIMycSp51234_2864.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciMSP164757:GHV3-2858-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JLS.

Entry informationi

Entry nameiMASZ_MYCSJ
AccessioniPrimary (citable) accession number: A3Q0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: January 7, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.