A3Q016 (KATG_MYCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Mjls_2713

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 749	749	Catalase-peroxidase HAMAP MF_01961		PRO_0000354841
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	281	1	Iron (heme axial ligand) By similarity
	Site	110	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	113 ↔ 240		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-266) By similarity
	Cross-link	240 ↔ 266		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3Q016 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 573DCEDAC5BE1391	FASTA	749	82,684
	10 20 30 40 50 60 MSSDTSDTRP PHSDSGTQSN SESENPIIDS PEPKAHAPLT NKDWWPEQVD VSVLHKQNDK 70 80 90 100 110 120 GNPLGEDFNY AEAFAQLDLE AFKRDVIEVI QTSQDWWPAD YGNYAGLFIR MSWHAAGTYR 130 140 150 160 170 180 IFDGRGGAGQ GSQRFAPLNS WPDNANLDKA RRLLWPIKQK YGNKISWADL IAYAGNAALE 190 200 210 220 230 240 QSGFKTAGFA FGREDIWEPE EMLWGQEDTW LGTDKRYGGT NEDKRELAEP FGATTMGLIY 250 260 270 280 290 300 VNPEGPEGKP DPLAAAHDIR ETFGRMAMND EETAALIVGG HTLGKTHGAA DVNVGPEPEG 310 320 330 340 350 360 APIEEQGLGW KCPFGTGNAN DTVTSGLEVI WTGTNSEWSN RYLEILYGNE WELTKSPAGA 370 380 390 400 410 420 WQFEAKNAEA TIPDPFGGPP RKPTMLVTDV SMREDPIYGQ ITRRWLDHPE EMDEAFAKAW 430 440 450 460 470 480 YKLMHRDMGP ISRYLGPWVA EPEIWQDPVP DVDHELVDES DIASLKSKVL ESGLTVQQLV 490 500 510 520 530 540 KTAWASASSF RGTDKRGGAN GARVRLEPQR SWEGNEPAEL AKVLPTLEQI QQDFNASASG 550 560 570 580 590 600 GKKISLADLI VLAGSAAVEK AAKDAGYEID VHFAPGRTDA SQEQTDVESF AVLETKADGF 610 620 630 640 650 660 RNYIRPGQKT SVEKLLVEKA YLLDLTAPEM TALLGGLRVL NVNHGGSKHG VFTNSPGALS 670 680 690 700 710 720 NDFFVNLLDM NTAWKPSENT ENVFEGRDRA TGEIKWTATA NDLVFGSNSV LRGIAEVYAQ 730 740 DDSKDKFVED FVAAWVKVMN NDRFDLEKF

References

[1]

"Complete sequence of Mycobacterium sp. JLS." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000580 Genomic DNA. Translation: ABN98493.1.
RefSeq	YP_001070984.1. NC_009077.1.
3D structure databases
ProteinModelPortal	A3Q016.
SMR	A3Q016. Positions 40-746.
ModBase	Search...
Protein-protein interaction databases
STRING	A3Q016.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000050812; EBMYCP00000049093; EBMYCG00000050807.
GeneID	4878427.
GenomeReviews	Gene locus Mjls_2713 in contig CP000580_GR.
KEGG	mjl:Mjls_2713.
PATRIC	18091589. VBIMycSp51234_2730.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
GeneTree	EBGT00050000017549.
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	A3Q016.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	MSP164757:MJLS_2713-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCSJ

Accession

Primary (citable) accession number: A3Q016

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program