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A3PYA8 (TYSY_MYCSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:Mjls_2099
OrganismMycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]
Taxonomic identifier164757 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008

Subunit structure

Homodimer By similarity. HAMAP MF_00008

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266Thymidylate synthase HAMAP MF_00008
PRO_1000000631

Sites

Active site1491 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3PYA8 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 2E77246F020A6543

FASTA26630,038
        10         20         30         40         50         60 
MPIPTPYEDL LRLVFERGTP KSDRTGTGTR SLFGHQMRYD LAAGFPLITT KKVHLKSVVY 

        70         80         90        100        110        120 
ELLWFLRGES NVRWLQEHGV TIWDEWASET GELGPVYGVQ WRSWPTPSGG HVDQISAAVD 

       130        140        150        160        170        180 
LLRTDPDSRR NIVSAWNVGE IPQMALPPCH AFFQFYVADG RLSCQLYQRS ADLFLGVPFN 

       190        200        210        220        230        240 
IASYALLTHM MAAQAGLGVG EFVWTGGDCH IYDNHVEQVT EQLSRDPRPY PELVLAQRDS 

       250        260 
IFDYTYEDVV VKNYDPHPAI KAPVAV

« Hide

References

[1]"Complete sequence of Mycobacterium sp. JLS."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JLS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000580 Genomic DNA. Translation: ABN97885.1.
RefSeqYP_001070376.1. NC_009077.1.

3D structure databases

ProteinModelPortalA3PYA8.
SMRA3PYA8. Positions 7-266.
ModBaseSearch...

Protein-protein interaction databases

STRINGA3PYA8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000050392; EBMYCP00000048673; EBMYCG00000050387.
GeneID4877819.
GenomeReviewsGene locus Mjls_2099 in contig CP000580_GR.
KEGGmjl:Mjls_2099.
PATRIC18090322. VBIMycSp51234_2101.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
GeneTreeEBGT00050000017116.
HOGENOMHBG588098.
OMARMALAPC.
PhylomeDBA3PYA8.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMSP164757:MJLS_2099-MONOMER.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KOK00560.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. Thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_MYCSJ
AccessionPrimary (citable) accession number: A3PYA8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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