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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Mycobacterium sp. (strain JLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi522MagnesiumUniRule annotation1
Metal bindingi528MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Mjls_2083
OrganismiMycobacterium sp. (strain JLS)
Taxonomic identifieri164757 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003297211 – 759Polyribonucleotide nucleotidyltransferaseAdd BLAST759

Interactioni

Protein-protein interaction databases

STRINGi164757.Mjls_2083.

Structurei

3D structure databases

ProteinModelPortaliA3PY93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini588 – 647KHUniRule annotationAdd BLAST60
Domaini659 – 728S1 motifUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG093I048M.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

A3PY93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVEIEDGV YESTAVIDNG SFGTRTIRFE TGRLAQQAAG AVVAYLDDET
60 70 80 90 100
MLLSATSASK SPKDHFDFFP LTIDVEERMY AAGRIPGSFF RREGRPSTDA
110 120 130 140 150
ILTCRLIDRP LRPTFVSGLR NEIQVVVTVM SLDPKDLYDV VAINAASAST
160 170 180 190 200
QIAGLPFSGP VGGVRVALID GTWVAFPTVE QLERAVFDMV VAGRKTADDV
210 220 230 240 250
AIMMVEAEAT DKVVELVAGG AQAPTEAVVA EGLEAAKPFI KVLCEAQQEL
260 270 280 290 300
AGRAAKPTAD YPLFPEYGED VYYAVASVAT DALSEALTIA GKEERNNRTD
310 320 330 340 350
EIKVEVLGRL ADQFAGREKE IGGAFRSLTK KLVRQRILTD HFRIDGRGVT
360 370 380 390 400
DIRALSAEVA IVPRAHGSAL FERGETQILG VTTLDMVKMA QQIDSLGPET
410 420 430 440 450
SKRYMHHYNF PPYSTGETGR VGSPKRREIG HGALAERALM PVLPSVEEFP
460 470 480 490 500
YAIRQVSEAL SSNGSTSMGS VCASTLSLLN AGVPLKAPVA GIAMGLVSDD
510 520 530 540 550
VEVDGKTERR FVTLTDILGA EDAFGDMDFK CAGTKDFVTA LQLDTKLDGI
560 570 580 590 600
PSQVLAGALA QAKDARITIL EVMAEAIDAP DEMSPYAPRI TTIKVPVDKI
610 620 630 640 650
GEVIGPKGKM INSITEETGA SISIEDDGTV FVGASNGEAA QAAIDKINAI
660 670 680 690 700
ANPQLPKIGE RFLGTVVKTT DFGAFVSLLP GRDGLVHISK LGRGKRIAKV
710 720 730 740 750
EDVAKVGDKL RVEIADIDNR GKISLVLVAE EEAAEASDNG SATPSDKAPA

TADATTAGN
Length:759
Mass (Da):80,676
Last modified:April 3, 2007 - v1
Checksum:i1DAE4E88C36EF12E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000580 Genomic DNA. Translation: ABN97870.1.
RefSeqiWP_011559498.1. NC_009077.1.

Genome annotation databases

EnsemblBacteriaiABN97870; ABN97870; Mjls_2083.
KEGGimjl:Mjls_2083.
PATRICi18090290. VBIMycSp51234_2085.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000580 Genomic DNA. Translation: ABN97870.1.
RefSeqiWP_011559498.1. NC_009077.1.

3D structure databases

ProteinModelPortaliA3PY93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi164757.Mjls_2083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN97870; ABN97870; Mjls_2083.
KEGGimjl:Mjls_2083.
PATRICi18090290. VBIMycSp51234_2085.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG093I048M.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_MYCSJ
AccessioniPrimary (citable) accession number: A3PY93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 3, 2007
Last modified: February 15, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.