A3PXW3 (RNH2_MYCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	Mjls_1952

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Ribonuclease HII HAMAP MF_00052_B		PRO_1000031165
Sites
	Metal binding	36	1	Divalent metal cation By similarity
	Metal binding	37	1	Divalent metal cation By similarity
	Metal binding	130	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3PXW3 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 6231F7F839332A76	FASTA	239	25,218
	10 20 30 40 50 60 MPASWPPRTV IRKASGLRTL ESALYRNGLG PVAGVDEVGR GACAGPLVVA ACVLGPNRLE 70 80 90 100 110 120 SLAALDDSKK LGEKERERLF PVIRRYALAY HVVFIPSGEV DRRGVHVANI EGMRRAVAGL 130 140 150 160 170 180 SVRPGYVLSD GFRVPGLPMP SLPVVGGDAA AACIAAASVL AKVSRDRLMV AMEREHPGYG 190 200 210 220 230 FAEHKGYSTP AHTAALAELG PCAQHRYSFI NVRRLVTAGT PQISGGLTDA EPGQCCELG

References

[1]

"Complete sequence of Mycobacterium sp. JLS." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000580 Genomic DNA. Translation: ABN97740.1.
RefSeq	YP_001070231.1. NC_009077.1.
3D structure databases
ProteinModelPortal	A3PXW3.
ModBase	Search...
Protein-protein interaction databases
STRING	A3PXW3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000047525; EBMYCP00000045806; EBMYCG00000047520.
GeneID	4877674.
GenomeReviews	Gene locus Mjls_1952 in contig CP000580_GR.
KEGG	mjl:Mjls_1952.
PATRIC	18090026. VBIMycSp51234_1953.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	A3PXW3.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	MSP164757:MJLS_1952-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCSJ

Accession

Primary (citable) accession number: A3PXW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program