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A3PV78 (RPOB_MYCSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene names
Name:rpoB
Ordered Locus Names:Mjls_0997
OrganismMycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]
Taxonomic identifier164757 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length1172 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_01321

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01321

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-dependent

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: HAMAP

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: HAMAP

ribonucleoside binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11721172DNA-directed RNA polymerase subunit beta HAMAP-Rule MF_01321
PRO_0000300350

Sequences

Sequence LengthMass (Da)Tools
A3PV78 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: F84A0673C6D63D81

FASTA1,172129,079
        10         20         30         40         50         60 
MLEGCILAVS SQSKSAKAIT NNSVPGAPNR ISFAKLREPL EVPGLLDVQT ESFDWLIGAD 

        70         80         90        100        110        120 
SWRQRATARG DVNPTGGLEE VLTELSPIED FSGSMSLSFS DPRFDEVKAP VDECKDKDMT 

       130        140        150        160        170        180 
YAAPLFVTAE FINNNTGEIK SQTVFMGDFP MMTEKGTFII NGTERVVVSQ LVRSPGVYFD 

       190        200        210        220        230        240 
ESIDKSTEKT LHSVKVIPGR GAWLEFDVDK RDTVGVRIDR KRRQPVTVLL KALGWTNEQI 

       250        260        270        280        290        300 
TERFGFSEIM MSTLEKDNTA GTDEALLDIY RKLRPGEPPT KESAQTLLEN LFFKEKRYDL 

       310        320        330        340        350        360 
ARVGRYKVNK KLGLNTDKPI TSSTLTEEDV VATIEYLVRL HEGQATMTVP GGVEVPVEVD 

       370        380        390        400        410        420 
DIDHFGNRRL RTVGELIQNQ IRVGLSRMER VVRERMTTQD VEAITPQTLI NIRPVVAAIK 

       430        440        450        460        470        480 
EFFGTSQLSQ FMDQNNPLSG LTHKRRLSAL GPGGLSRERA GLEVRDVHSS HYGRMCPIET 

       490        500        510        520        530        540 
PEGPNIGLIG SLSVYARVNP FGFIETPYRK VENGVVTDQI DYLTADEEDR HVVAQANSPL 

       550        560        570        580        590        600 
DDEGHFTEDR VLVRRKGGEV EFVSATEVDY MDVSPRQMVS VATAMIPFLE HDDANRALMG 

       610        620        630        640        650        660 
ANMQRQAVPL VRSEAPLVGT GMELRAAIDA GDVVVSEKAG VVEEVSADYI TVMADDGTRH 

       670        680        690        700        710        720 
TYRMRKFARS NHGTCANQRP IVDAGQRVEA GQVVADGPCT QNGEMALGKN LLVAIMPWEG 

       730        740        750        760        770        780 
HNYEDAIILS NRLVEEDVLT SIHIEEHEID ARDTKLGAEE ITRDIPNVSD EVLADLDERG 

       790        800        810        820        830        840 
IVRIGAEVRD GDILVGKVTP KGETELTPEE RLLRAIFGEK AREVRDTSLK VPHGESGKVI 

       850        860        870        880        890        900 
GIRVFSREDD DELPAGVNEL VRVYVAQKRK ISDGDKLAGR HGNKGVIGKI LPVEDMPFLP 

       910        920        930        940        950        960 
DGTPVDIILN THGVPRRMNI GQILETHLGW VAKAGWNINV AGADGVPDWA EKLPEELYSA 

       970        980        990       1000       1010       1020 
PSDSIVATPV FDGARENELS GLLASTLPNR DGDVMVNEDG KAELFDGRSG EPFPYPVTVG 

      1030       1040       1050       1060       1070       1080 
YMYILKLHHL VDDKIHARST GPYSMITQQP LGGKAQFGGQ RFGEMECWAM QAYGAAYTLQ 

      1090       1100       1110       1120       1130       1140 
ELLTIKSDDT VGRVKVYEAI VKGENIPEPG IPESFKVLLK ELQSLCLNVE VLSSDGAAIE 

      1150       1160       1170 
MRDGDDEDLE RAAANLGINL SRNESASVED LA

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References

[1]"Complete sequence of Mycobacterium sp. JLS."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JLS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000580 Genomic DNA. Translation: ABN96805.1.
RefSeqYP_001069296.1. NC_009077.1.

3D structure databases

ProteinModelPortalA3PV78.
ModBaseSearch...

Protein-protein interaction databases

STRING164757.Mjls_0997.

Proteomic databases

PRIDEA3PV78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN96805; ABN96805; Mjls_0997.
GeneID4876738.
KEGGmjl:Mjls_0997.
PATRIC18088101. VBIMycSp51234_0995.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0085.
HOGENOMHOG000218611.
KOK03043.
OMAFMTWEGY.
ProtClustDBPRK00405.

Enzyme and pathway databases

BioCycMSP164757:GHV3-1054-MONOMER.

Family and domain databases

Gene3D2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
HAMAPMF_01321. RNApol_bact_RpoB.
InterProIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERPTHR20856. PTHR20856. 1 hit.
PTHR20856:SF3. PTHR20856:SF3. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR02013. rpoB. 1 hit.
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOB_MYCSJ
AccessionPrimary (citable) accession number: A3PV78
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: April 3, 2007
Last modified: May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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