A3PUK0 (HTPX_MYCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protease HtpX homolog
EC=3.4.24.-

Gene names

Name:	htpX
Ordered Locus Names:	Mjls_0767

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00188
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00188.
Sequence similarities	Belongs to the peptidase M48B family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Protease HtpX homolog HAMAP-Rule MF_00188		PRO_1000020890
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
Sites
	Active site	136	1	By similarity
	Metal binding	135	1	Zinc; catalytic By similarity
	Metal binding	139	1	Zinc; catalytic By similarity
	Metal binding	206	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3PUK0 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 1E80266055A97C33	FASTA	291	31,372
	10 20 30 40 50 60 MTWNPHANRF KTFLLLVGMS ALIVFVGSLF GRSIMALAVL FAVGMNVYVY FNSDKLALKA 70 80 90 100 110 120 MHAQPVSELQ APVMYRIVRE LSNAAHQPMP RLYISDTANP NAFATGRNPR NSAVCCTTGI 130 140 150 160 170 180 LQILNERELR AVLGHELSHV YNRDILISCV AGAMASVITA LANIALFAGM FGGNREGTNP 190 200 210 220 230 240 FALLLVSFLG PIAATVVRLA VSRSREYQAD QSGAELTGDP LALASALRKI SGGVEAAPLP 250 260 270 280 290 PQPQLADQAH LMIASPFRSG EKIGKLFSTH PPMADRIRRL EEMAGRGPGL Y

References

[1]

"Complete sequence of Mycobacterium sp. JLS." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000580 Genomic DNA. Translation: ABN96577.1.
RefSeq	YP_001069068.1. NC_009077.1.
3D structure databases
ProteinModelPortal	A3PUK0.
ModBase	Search...
Protein-protein interaction databases
STRING	164757.Mjls_0767.
Protein family/group databases
MEROPS	M48.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN96577; ABN96577; Mjls_0767.
GeneID	4876510.
KEGG	mjl:Mjls_0767.
PATRIC	18087657. VBIMycSp51234_0777.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0501.
HOGENOM	HOG000227301.
KO	K03799.
OMA	RRGAEIC.
ProtClustDB	PRK03072.
Enzyme and pathway databases
BioCyc	MSP164757:GHV3-771-MONOMER.
Family and domain databases
HAMAP	MF_00188. Peptidase_M48.
InterPro	IPR022919. Pept_M48_protease_HtpX. IPR001915. Peptidase_M48. [Graphical view]
Pfam	PF01435. Peptidase_M48. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HTPX_MYCSJ

Accession

Primary (citable) accession number: A3PUK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	May 29, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program