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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium sp. (strain JLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei211D-inositol 3-phosphateUniRule annotation1
Binding sitei35UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei901D-inositol 3-phosphateUniRule annotation1
Binding sitei1231D-inositol 3-phosphateUniRule annotation1
Binding sitei1471D-inositol 3-phosphateUniRule annotation1
Binding sitei1671D-inositol 3-phosphateUniRule annotation1
Binding sitei241UDP-GlcNAcUniRule annotation1
Binding sitei246UDP-GlcNAcUniRule annotation1
Binding sitei299UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi308Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi309Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi311Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei321UDP-GlcNAcUniRule annotation1
Binding sitei329UDP-GlcNAcUniRule annotation1
Metal bindingi335MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandMagnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Mjls_0650
OrganismiMycobacterium sp. (strain JLS)
Taxonomic identifieri164757 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001381 – 439D-inositol 3-phosphate glycosyltransferaseAdd BLAST439

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA3PU84.
SMRiA3PU84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 28UDP-GlcNAc bindingUniRule annotation2
Regioni32 – 371D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiView protein in InterPro
IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
PfamiView protein in Pfam
PF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A3PU84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLATDQLGR PPQRVAVLSV HTSPLAQPGT GDAGGMNVYV LQSALHMARR
60 70 80 90 100
GVEVEIFTRA TTSADPPVVR VAPGVLVRNV VAGPFEGLDK YDLPTQLCAF
110 120 130 140 150
TAGVLRAEAT HEPGYYDIVH SHYWLSGQVG WLARDRWAVP LVHTAHTLAA
160 170 180 190 200
VKNAALAEGD SPEPPLRAVG EQQVVDEADR LIVNTELEAE QLVSLHNADP
210 220 230 240 250
SRIDVVHPGV DLDTFTPGDQ AAARAALGLD PRETVVAFVG RIQPLKAPDI
260 270 280 290 300
LLRAAAKLPD VRVLVAGGPS GSGLAAPDNL VALADELGIS ERVTFLPPQS
310 320 330 340 350
REDLVRVYRA ADLVAVPSYS ESFGLVAVEA QACGTPVVAA AVGGLPVAVR
360 370 380 390 400
DGVTGALVDG HDVGDWAHTI DSLLSRGPAT MRRAAVEHAA TFSWAHTVDD
410 420 430
LLASYGRAIS DYRDRHPHAD ETLSRRTARR FSRRRGVRA
Length:439
Mass (Da):46,734
Last modified:April 3, 2007 - v1
Checksum:iFC55BF85068630F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000580 Genomic DNA. Translation: ABN96461.1.
RefSeqiWP_011854536.1. NC_009077.1.

Genome annotation databases

EnsemblBacteriaiABN96461; ABN96461; Mjls_0650.
KEGGimjl:Mjls_0650.

Similar proteinsi

Entry informationi

Entry nameiMSHA_MYCSJ
AccessioniPrimary (citable) accession number: A3PU84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: April 3, 2007
Last modified: September 27, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families