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A3PMN4 (SYL_RHOS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Rsph17029_2498
OrganismRhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) [Complete proteome] [HAMAP]
Taxonomic identifier349101 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length847 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 847847Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009413

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3PMN4 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 33C63C56B841C53B

FASTA84794,165
        10         20         30         40         50         60 
MSRYDPAATE SRWQAAWDAA GVFTARHDPA RPKYYVLEMF PYPSGRIHMG HVRNYTMGDV 

        70         80         90        100        110        120 
VARQKAAAGF SVLHPMGWDA FGMPAENAAM ERGGHPKDWT YGNIADMRAQ MKPLGLSIDW 

       130        140        150        160        170        180 
SREFATCDPE YYGQQQAMFI DMMEAGLVYR KNAVVNWDPV DMTVLANEQV IDGKGWRSGA 

       190        200        210        220        230        240 
PVVRRELTQW FFRISDYAGE LLEALDTLKD WPEKVRLMQA NWIGQSRGLQ FAFSMAGAPE 

       250        260        270        280        290        300 
GFDRLEVYTT RPDTLMGASF AAISPDHPLA RHLERHDAEV AEFVAECRRV GTSEEALEKA 

       310        320        330        340        350        360 
EKKGFDTGLR VRHPFDTAWE LPVYIANFIL MDYGTGAIFG CPAHDQRDFE FATKYGLPIR 

       370        380        390        400        410        420 
PVFLPEDTEE TALAEAFVPM KSERVHYIRG FAGAEVQTGE EGVAAAIDFC ESQGVGRGVT 

       430        440        450        460        470        480 
NYRLRDWGIS RQRYWGCPIP VIHCETCGVV PEAKENLPVR LPDDVSFDVP GNPLDRHPTW 

       490        500        510        520        530        540 
RDCTCPKCGA KARRETDTMD TFVDSSWYYA RFTAPRAATP TDAEEADYWM NVDQYIGGIE 

       550        560        570        580        590        600 
HAILHLLYSR FFARAMQKTG HLPAKAIEPF NALFTQGMVT HEIYLTRDAA GRPVYHLPED 

       610        620        630        640        650        660 
VTDGRLADGT PVEIIPSAKM SKSKKNVVDP MNIIRQFGAD TARWFVMSDS PPERDVEWTA 

       670        680        690        700        710        720 
SGAEAASKHL HRVWRLADEI SRADGEANAE DGALDKATAR AIAEVTQGVE GFAFNKAIAK 

       730        740        750        760        770        780 
LYEFTNTLSR SGAGAEAKKR AMRTMAQLMS PMVPHLAEEV WAMLGGEGLV AQAAWPKADP 

       790        800        810        820        830        840 
ALLIDDTVTL PIQVNGKRRG EITVPKEMAA SEVEKLVLAD EAVQRALGGA APKKLIVVPG 


RIVNVVI 

« Hide

References

[1]"Complete sequence of chromosome 1 of Rhodobacter sphaeroides ATCC 17029."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17029 / ATH 2.4.9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000577 Genomic DNA. Translation: ABN77600.1.
RefSeqYP_001044372.1. NC_009049.1.

3D structure databases

ProteinModelPortalA3PMN4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349101.Rsph17029_2498.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN77600; ABN77600; Rsph17029_2498.
GeneID4895451.
KEGGrsh:Rsph17029_2498.
PATRIC23172873. VBIRhoSph114483_2728.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycRSPH349101:GHC8-2537-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RHOS1
AccessionPrimary (citable) accession number: A3PMN4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries