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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciRSPH349101:GHC8-1307-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:Rsph17029_1286
OrganismiRhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)
Taxonomic identifieri349101 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002606 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_1000063925Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA3PJ80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3PJ80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAECLHIVGA GMAGSEAAWQ AAEMGVPVVL HEMRPKVGTF AHRTGQFAEM
60 70 80 90 100
VCSNSFRSDD DERNAVGLLH WEMRAARGLI MEMAAAHRLP AGGALAVDRD
110 120 130 140 150
PFAESVTARL RAHPLISVVE EEVAELPSSG NWIVATGPLT SSALAESLRA
160 170 180 190 200
LTGAEALAFF DAIAPIVYAE TIDMEVAWRQ SRYDKGETED ERTAYINCPM
210 220 230 240 250
NREQYEAFID ALLAAEKTEF HEGETAGYFD GCLPIEVMAE RGRETLRHGP
260 270 280 290 300
MKPVGLTNSH RPAEKAYAVV QLRRDNKLGT LYNIVGFQTK MKYGAQTAVF
310 320 330 340 350
KMIPGLENAS FARLGGIHRN TFLNSPTLLD DRMRLKLRPN IRFAGQVTGV
360 370 380 390 400
EGYVESAAMG LLAGRMAAAE ILGRDLPPPP PETAMGALVT HITGGAEAKS
410 420 430 440
FQPMNVNFGL FPPIDARGGR RGRKDRYKAY TDRAKAAFTE WLGA
Length:444
Mass (Da):48,424
Last modified:April 3, 2007 - v1
Checksum:i09D5DABB5EF7C3A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000577 Genomic DNA. Translation: ABN76396.1.
RefSeqiWP_011840920.1. NC_009049.1.

Genome annotation databases

EnsemblBacteriaiABN76396; ABN76396; Rsph17029_1286.
KEGGirsh:Rsph17029_1286.
PATRICi23170313. VBIRhoSph114483_1466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000577 Genomic DNA. Translation: ABN76396.1.
RefSeqiWP_011840920.1. NC_009049.1.

3D structure databases

ProteinModelPortaliA3PJ80.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN76396; ABN76396; Rsph17029_1286.
KEGGirsh:Rsph17029_1286.
PATRICi23170313. VBIRhoSph114483_1466.

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciRSPH349101:GHC8-1307-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides ATCC 17029."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.
    , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S.
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17029 / ATH 2.4.9.

Entry informationi

Entry nameiTRMFO_RHOS1
AccessioniPrimary (citable) accession number: A3PJ80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 3, 2007
Last modified: January 20, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.