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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091NucleophileUniRule annotation
Active sitei462 – 4621Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciRSPH349101:GHC8-1131-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Rsph17029_1111
OrganismiRhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)
Taxonomic identifieri349101 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002606 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7287281,4-alpha-glucan branching enzyme GlgBPRO_1000044996Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349101.Rsph17029_1111.

Structurei

3D structure databases

ProteinModelPortaliA3PIQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A3PIQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVTSKPGEL VPESDVAAIM RGTHGDPFRV LGMHGGGGAP LSVRVFAPQA
60 70 80 90 100
AEVAVLGQGG KVLASLERIG AEGFFAGTVP GEEKFPYRLR FVSGPHEWEA
110 120 130 140 150
DDPYRFPEVL GELDEYLLGE GRHYQLYTRL GAHPAEIEGV QGVSFAVWAP
160 170 180 190 200
NARRVSVVGA FNAWDGRRHP MRKRIGVGVW ELFVPGLHTG DLYKYEILGP
210 220 230 240 250
SGERLPLKSD PLSFAQEAPP ATASVVHGLP EAEWHDAGWM QERESRQRRD
260 270 280 290 300
APISIYEVHA GSWRQGLDYD ALAEELSAYV REMGFTHVEF LPISEHPFTG
310 320 330 340 350
SWGYQPIGLF APTARFGPPE GFARLVDRLH RDGIGVILDW VPAHFPSDAH
360 370 380 390 400
GLANFDGTHL YDHADPRQGF HRDWNTQIYN FGRQEVANFL QASALFWLDR
410 420 430 440 450
YHVDALRVDA VASMLYLDYS RNAGEWVPNR HGGRENLEAI DFLRGVNERV
460 470 480 490 500
RLDHPGCITI AEESTAFPQV SRPVEDGGLG FGFKWNMGWM HDTLGYFRRD
510 520 530 540 550
PIHRKHHQND LTFGMVYAYS EDFVLPLSHD EVVHGKGSLI GQMAGDRWQK
560 570 580 590 600
FANLRAYFGF MWAHPGKKLL FMGGEFAQER EWNHDASLDW HLLDDPSHAG
610 620 630 640 650
MKRLVADLNR EYRKRPALHR MDCDPEGFEW IDAGDSENSV LSFLRKAPGE
660 670 680 690 700
KPVLAVCNLT PVVRSDYRIG VPEGGEWREI LNSDAAIYGG SDVGNPGGLQ
710 720
AEEVSWHGRP ASLRLTLPPL ATIFVTPA
Length:728
Mass (Da):81,398
Last modified:April 3, 2007 - v1
Checksum:iD707D8F34B96704C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000577 Genomic DNA. Translation: ABN76221.1.
RefSeqiWP_011840807.1. NC_009049.1.
YP_001042993.1. NC_009049.1.

Genome annotation databases

EnsemblBacteriaiABN76221; ABN76221; Rsph17029_1111.
KEGGirsh:Rsph17029_1111.
PATRICi23169950. VBIRhoSph114483_1286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000577 Genomic DNA. Translation: ABN76221.1.
RefSeqiWP_011840807.1. NC_009049.1.
YP_001042993.1. NC_009049.1.

3D structure databases

ProteinModelPortaliA3PIQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349101.Rsph17029_1111.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN76221; ABN76221; Rsph17029_1111.
KEGGirsh:Rsph17029_1111.
PATRICi23169950. VBIRhoSph114483_1286.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciRSPH349101:GHC8-1131-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides ATCC 17029."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.
    , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S.
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17029 / ATH 2.4.9.

Entry informationi

Entry nameiGLGB_RHOS1
AccessioniPrimary (citable) accession number: A3PIQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: April 29, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.