A3PGE9 (ISPH_RHOS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Rsph17029_0299

Organism

Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) [Complete proteome] [HAMAP]

Taxonomic identifier

349101 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021170

Sequences

	Sequence		Length	Mass (Da)
	A3PGE9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 844BE2CA78D6E04E	FASTA	319	34,676
	10 20 30 40 50 60 MTMPPLTLYL AAPRGFCAGV DRAIKIVEMA LEKWGAPVYV RHEIVHNKFV VDRLRDMGAV 70 80 90 100 110 120 FVEELDEAPT DRPVIFSAHG VPKAIPAEAE RRNMVYVDAT CPLVSKVHLE AERHHENGLQ 130 140 150 160 170 180 MIMIGHAGHP ETVGTMGQLP EGEVLLVETV EDVAGLEVRD PERLAYITQT TLSIDDTAAI 190 200 210 220 230 240 VAALRERFPA IAIPRKEDIC YATTNRQGAV KAIAGRIDAL LVIGAPNSSN SKRLVEVGRA 250 260 270 280 290 300 AGCRVAQLVQ RATDIDWEAL QGATSVGVAA GASAPEVLVD EVIAAFRARF DTTVKAVETV 310 KERVEFKVPR ILREPAETP

References

[1]

"Complete sequence of chromosome 1 of Rhodobacter sphaeroides ATCC 17029." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17029 / ATH 2.4.9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000577 Genomic DNA. Translation: ABN75415.1.
RefSeq	YP_001042187.1. NC_009049.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3PGE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4897711.
GenomeReviews	Gene locus Rsph17029_0299 in contig CP000577_GR.
KEGG	rsh:Rsph17029_0299.
PATRIC	23168205. VBIRhoSph114483_0423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	WGAPVYV.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	RSPH349101:RSPH17029_0299-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_RHOS1

Accession

Primary (citable) accession number: A3PGE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program