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Protein

UvrABC system protein B

Gene

uvrB

Organism
Prochlorococcus marinus (strain MIT 9301)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:P9301_18391
OrganismiProchlorococcus marinus (strain MIT 9301)
Taxonomic identifieri167546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000001430 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000779081 – 679UvrABC system protein BAdd BLAST679

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi167546.P9301_18391.

Structurei

3D structure databases

ProteinModelPortaliA3PFD7.
SMRiA3PFD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 176Helicase ATP-bindingUniRule annotationAdd BLAST152
Domaini429 – 583Helicase C-terminalUniRule annotationAdd BLAST155
Domaini639 – 674UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi91 – 114Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiRYMHSEI.
OrthoDBiPOG091H02LK.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

A3PFD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNYKLQAPY EPNGDQPEAI KKLVKGVNTG KEFQTLLGAT GTGKTFTIAN
60 70 80 90 100
VIQQTGRPAL VLAHNKTLAA QLCNELREFF PKNAVEYFIS YYDYYQPEAY
110 120 130 140 150
VPVSDTYIAK TASINEEIDM LRHSATRSLF ERKDVIVVAS ISCIYGLGIP
160 170 180 190 200
SEYLKAAVKF EVGKSINLRS YLRSLVENQY TRNDIEITRG RFRIKGDVLE
210 220 230 240 250
IGPAYEDRLI RIELFGDEVE AIRYVDPTTG EILESLEQVS VYPAKHFVTP
260 270 280 290 300
KERLESAISA IRSELKTQLD KFTYEGKLLE AQRLEQRTKY DLEMLKEVGY
310 320 330 340 350
CNGVENYARH LSGREEGSPP ECLIDYFPKD WLLVVDESHV TCPQLHAMYN
360 370 380 390 400
GDQSRKKVLI DHGFRLPSAA DNRPLKCEEF WEKSKQTLFI SATPGQWELD
410 420 430 440 450
QCDGEFIEQV IRPTGVLDPV IDVRPSEGQI EDLLSEIRIR AEKNQRVLVT
460 470 480 490 500
TLTKRMAEDL TDFLSENKVR VRYLHSEIHS IERIEIIQDL RMGEYDVLVG
510 520 530 540 550
VNLLREGLDL PEVSLVAILD ADKEGFLRAE RSLIQTIGRA ARHVEGVALL
560 570 580 590 600
YADNFTDSMK RAISETERRR TIQKKYNQVN GITPKPAGKK IENSILSFLE
610 620 630 640 650
LSRKLDAGGL SKDLINIVNN KTDAILGSSD NQCLLEELPD LIEKLEIKMK
660 670
DAAKELNFEE AANLRDRIKK LRQKLARNN
Length:679
Mass (Da):77,317
Last modified:April 3, 2007 - v1
Checksum:i453858A89B5AA1B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000576 Genomic DNA. Translation: ABO18462.1.
RefSeqiWP_011863746.1. NC_009091.1.

Genome annotation databases

EnsemblBacteriaiABO18462; ABO18462; P9301_18391.
GeneIDi4911161.
KEGGipmg:P9301_18391.

Similar proteinsi

Entry informationi

Entry nameiUVRB_PROM0
AccessioniPrimary (citable) accession number: A3PFD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 3, 2007
Last modified: October 25, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families