A3PED9 (MIAB_PROM0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
EC=2.-.-.-

Alternative name(s):
tRNA-i(6)A37 methylthiotransferase

Gene names

Name:	miaB
Ordered Locus Names:	P9301_14911

Organism

Prochlorococcus marinus (strain MIT 9301) [Complete proteome] [HAMAP]

Taxonomic identifier

167546 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Prochlorales › Prochlorococcaceae › Prochlorococcus › Prochlorococcus marinus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01864
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01864.
Sequence similarities	Belongs to the methylthiotransferase family. MiaB subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864		PRO_0000374447
Regions
	Domain	19 – 135	117	MTTase N-terminal
	Domain	396 – 464	69	TRAM
Sites
	Metal binding	28	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	64	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	98	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	170	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	174	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	177	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3PED9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: A71367685D741370	FASTA	464	52,724
	10 20 30 40 50 60 MLTKTKSDEI KTQKDSTTGS YWITTFGCQM NKADSERMAG TLEKMGYTRA DNELNADLVL 70 80 90 100 110 120 YNTCTIRDNA EQKVYSFLGR QAKRKHKTPR LKLVVAGCLA QQEGESLLRR VPELDLVMGP 130 140 150 160 170 180 QHVNNLENLL GKVDLGNQVV ATEETFISED ITSARRESSI CGWVNIIYGC NERCSYCVVP 190 200 210 220 230 240 SVRGKEQSRY PNAIKSEIQK LADDNFKEIT LLGQNIDAYG RDLPGTTKEG RKENTLTDLL 250 260 270 280 290 300 YYIHDVKGIR RIRFATSHPR YFSKRLIQAC YELDKVCEHF HIPFQSGNDE ILKQMSRGYS 310 320 330 340 350 360 IKKYKNIIEN IRSLMPDASI TADAIVAFPG ETEKQYQDTL KLISEIGFDQ VNTAAYSARP 370 380 390 400 410 420 NTPAAVWTNQ LSEEVKKARL QEINDLVEKT ARSRNQRYID NIESVLIEGL NPKNSSQIMG 430 440 450 460 RTRTNRLTFV EIPKNINFNF SLGDEINVRI NEARPFSLTG ELSL

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000576 Genomic DNA. Translation: ABO18114.1.
RefSeq	YP_001091715.1. NC_009091.1.
3D structure databases
ProteinModelPortal	A3PED9.
ModBase	Search...
Protein-protein interaction databases
STRING	167546.P9301_14911.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABO18114; ABO18114; P9301_14911.
GeneID	4912718.
KEGG	pmg:P9301_14911.
PATRIC	22996495. VBIProMar103344_1424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HOG000224767.
KO	K06168.
OMA	QHERISA.
ProtClustDB	PRK14333.
Enzyme and pathway databases
BioCyc	PMAR167546:GH1Y-1529-MONOMER.
Family and domain databases
Gene3D	3.80.30.20. 1 hit.
HAMAP	MF_01864. tRNA_metthiotr_MiaB.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR006463. MiaB_methiolase. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF01938. TRAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01574. miaB-methiolase. 1 hit. TIGR00089. TIGR00089. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIAB_PROM0

Accession

Primary (citable) accession number: A3PED9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 3, 2007
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program