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A3PDJ8

- BIOB_PROM0

UniProt

A3PDJ8 - BIOB_PROM0

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Protein
Biotin synthase
Gene
bioB, P9301_12001
Organism
Prochlorococcus marinus (strain MIT 9301)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi105 – 1051Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi197 – 1971Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi269 – 2691Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMAR167546:GH1Y-1230-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:P9301_12001
OrganismiProchlorococcus marinus (strain MIT 9301)
Taxonomic identifieri167546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001430: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Biotin synthaseUniRule annotation
PRO_0000381536Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi167546.P9301_12001.

Structurei

3D structure databases

ProteinModelPortaliA3PDJ8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A3PDJ8-1 [UniParc]FASTAAdd to Basket

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MANSNNQLLK EIRYDWNREE ILQILNMPLI DLMWEAQIVH RKFNKYDIQL    50
ASLFSVKTGG CEENCSYCSQ SIYSASEIKS HPQFQVEEVL ARAKVAKNEG 100
ADRFCMGWAW REIRDGKSFN AMLEMVSGVR DLGMEACVTA GMLTEEQASR 150
LADAGLTAYN HNLDTSPEHY KNIITTRTYQ DRLDTIKRVR NAGINVCCGG 200
IIGLGETNGD RASLLEVLSN MNPHPESVPI NSLVAIEGTG LEDTQEIDSI 250
EMIRMIATAR ILMPESKIRL SAGREKLTKE AQILCFQCGA NSIFYGDELL 300
TTSNPSFQSD RKLLKEVGVS FNKDFETCEK TLSSL 335
Length:335
Mass (Da):37,544
Last modified:July 28, 2009 - v2
Checksum:iD60E8AD1D2C160FF
GO

Sequence cautioni

The sequence ABO17823.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000576 Genomic DNA. Translation: ABO17823.1. Different initiation.
RefSeqiYP_001091424.1. NC_009091.1.

Genome annotation databases

EnsemblBacteriaiABO17823; ABO17823; P9301_12001.
GeneIDi4911502.
KEGGipmg:P9301_12001.
PATRICi22995931. VBIProMar103344_1149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000576 Genomic DNA. Translation: ABO17823.1 . Different initiation.
RefSeqi YP_001091424.1. NC_009091.1.

3D structure databases

ProteinModelPortali A3PDJ8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167546.P9301_12001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO17823 ; ABO17823 ; P9301_12001 .
GeneIDi 4911502.
KEGGi pmg:P9301_12001.
PATRICi 22995931. VBIProMar103344_1149.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PMAR167546:GH1Y-1230-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9301.

Entry informationi

Entry nameiBIOB_PROM0
AccessioniPrimary (citable) accession number: A3PDJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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