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A3PDJ8

- BIOB_PROM0

UniProt

A3PDJ8 - BIOB_PROM0

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Protein

Biotin synthase

Gene

bioB

Organism
Prochlorococcus marinus (strain MIT 9301)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi105 – 1051Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi137 – 1371Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi197 – 1971Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi269 – 2691Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMAR167546:GH1Y-1230-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:P9301_12001
OrganismiProchlorococcus marinus (strain MIT 9301)
Taxonomic identifieri167546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001430: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Biotin synthasePRO_0000381536Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi167546.P9301_12001.

Structurei

3D structure databases

ProteinModelPortaliA3PDJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A3PDJ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANSNNQLLK EIRYDWNREE ILQILNMPLI DLMWEAQIVH RKFNKYDIQL
60 70 80 90 100
ASLFSVKTGG CEENCSYCSQ SIYSASEIKS HPQFQVEEVL ARAKVAKNEG
110 120 130 140 150
ADRFCMGWAW REIRDGKSFN AMLEMVSGVR DLGMEACVTA GMLTEEQASR
160 170 180 190 200
LADAGLTAYN HNLDTSPEHY KNIITTRTYQ DRLDTIKRVR NAGINVCCGG
210 220 230 240 250
IIGLGETNGD RASLLEVLSN MNPHPESVPI NSLVAIEGTG LEDTQEIDSI
260 270 280 290 300
EMIRMIATAR ILMPESKIRL SAGREKLTKE AQILCFQCGA NSIFYGDELL
310 320 330
TTSNPSFQSD RKLLKEVGVS FNKDFETCEK TLSSL
Length:335
Mass (Da):37,544
Last modified:July 28, 2009 - v2
Checksum:iD60E8AD1D2C160FF
GO

Sequence cautioni

The sequence ABO17823.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000576 Genomic DNA. Translation: ABO17823.1. Different initiation.
RefSeqiYP_001091424.1. NC_009091.1.

Genome annotation databases

EnsemblBacteriaiABO17823; ABO17823; P9301_12001.
GeneIDi4911502.
KEGGipmg:P9301_12001.
PATRICi22995931. VBIProMar103344_1149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000576 Genomic DNA. Translation: ABO17823.1 . Different initiation.
RefSeqi YP_001091424.1. NC_009091.1.

3D structure databases

ProteinModelPortali A3PDJ8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167546.P9301_12001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO17823 ; ABO17823 ; P9301_12001 .
GeneIDi 4911502.
KEGGi pmg:P9301_12001.
PATRICi 22995931. VBIProMar103344_1149.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PMAR167546:GH1Y-1230-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9301.

Entry informationi

Entry nameiBIOB_PROM0
AccessioniPrimary (citable) accession number: A3PDJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: October 1, 2014
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3