Reviewed, UniProtKB/Swiss-Prot A3PD22 (ACSF_PROM0)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	P9301_10241

Organism

Prochlorococcus marinus (strain MIT 9301) [Complete proteome] [HAMAP]

Taxonomic identifier

167546 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_1000070545

Sequences

	Sequence		Length	Mass (Da)
	A3PD22-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 6D736E73A1CADCFC	FASTA	390	45,600
	10 20 30 40 50 60 MSQSTIESTN KKEINKGKAP AKETILSPRF YTTDFEAMEN MDLSINEEEL EAICEEFRKD 70 80 90 100 110 120 YNRHHFVRNS EFEGAAEKLD PETRELFVDF LEGSCTSEFS GFLLYKELSK RIKDKNPLLA 130 140 150 160 170 180 ECFAHMARDE ARHAGFLNKS MSDFGLQLDL GFLTANKDYT YFPPRSIFYA TYLSEKIGYW 190 200 210 220 230 240 RYIAIYRHLE KNPNSKIFPL FNYFENWCQD ENRHGDFFDA LMKAQPRTVK SLSQKITIGG 250 260 270 280 290 300 STFTHPLFDY FHRFRYFLNN LPLTSKLWSR FFLLAVFATM YARDLGIKKD FYSSLGLDAR 310 320 330 340 350 360 DYDQFVINKT NETAARVFPV VMDVNNKSFY GRLDKIVENN KILSDIASGT GNKVSKTFRK 370 380 390 VPKYLSNGYQ LLRLYLLKPL DSKDYQPSIR

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000576 Genomic DNA. Translation: ABO17647.1.
RefSeq	YP_001091248.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4912750.
GenomeReviews	Gene locus P9301_10241 in contig CP000576_GR.
KEGG	pmg:P9301_10241.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PROM0

Accession

Primary (citable) accession number: A3PD22

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)