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A3PCW8 (SYL_PROM0) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:P9301_09701
OrganismProchlorococcus marinus (strain MIT 9301) [Complete proteome] [HAMAP]
Taxonomic identifier167546 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 856856Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009392

Regions

Motif53 – 6311"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3PCW8 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: CA4C08EB051D04E3

FASTA85698,780
        10         20         30         40         50         60 
MISPDQQYET DTNLYNPSEI EKKWQSIWTE NNLYKTDELT DNSDKFYALS MFPYPSGNLH 

        70         80         90        100        110        120 
MGHVRNYVIT DLIARFQRFK GKSVLHPMGW DAFGLPAENA AIERGISPSV WTKKNISHMK 

       130        140        150        160        170        180 
SQLKLLGLSV DWDREFATCD ENYYVWTQYL FLELYKAGLV YQKESEVNWD PIDNTVLANE 

       190        200        210        220        230        240 
QVDSEGKSWR SGAIVEKKLL KQWFLRITNY ADELLKDLEK LDNWPERVKI MQDNWIGKSI 

       250        260        270        280        290        300 
GTNINFNINT HPEKKLTVFT TRPDTLFGVT YLAISVNHSL IKNISDQETI QHIENLKKYL 

       310        320        330        340        350        360 
KKNKNNELEK IGIKTSLIAI NPVNSEPIPI WVASYVLDEY GTGAVMGVPA HDLRDFEFAK 

       370        380        390        400        410        420 
KNNIDIKQVI IKDKSEQNKE LDEAYVENGY LINSNQYDGV ANTIAKLKIS EEGVNNRWAE 

       430        440        450        460        470        480 
NKTQYRLRDW LISRQRYWGC PIPIVNCKKC GSVPLNQSEL PVALPKDIDI SANKINALGD 

       490        500        510        520        530        540 
NKNWVNTTCP KCGIAAKKET DTMDTFMCSS WYFLRYPSSK CPNKPFEKIE INKWLPVDQY 

       550        560        570        580        590        600 
VGGVEHAILH LLYARFFTKA LRDNQLFEID EPFKKLLTQG MVQAAAYKNN KTGKYVSPSD 

       610        620        630        640        650        660 
INDLSNPTDP IDNTKLEILF EKMSKSKYNG IDPESVIKKY GADTARMFIL FKAPPEKDLE 

       670        680        690        700        710        720 
WGDTDVEGQF RFLSRIWKLY INCAKNVNIK SNSYPDKEKS LIKSMNIAIK EISNDILNNQ 

       730        740        750        760        770        780 
FNTAISELMK FYNSLSNSIN DVNNNLKIDA LKTFCILLAP FAPHIAEEIW HLIGFKKSVH 

       790        800        810        820        830        840 
LEHWPSFNAE ALKEDSYELV IQVNGKVREK VNINHDMNED QIKELTLKRP NILKWTKDKE 

       850 
IRKIIIVKGK IMNIVV 

« Hide

References

[1]"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus."
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.
PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MIT 9301.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000576 Genomic DNA. Translation: ABO17593.1.
RefSeqYP_001091194.1. NC_009091.1.

3D structure databases

ProteinModelPortalA3PCW8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167546.P9301_09701.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO17593; ABO17593; P9301_09701.
GeneID4911693.
KEGGpmg:P9301_09701.
PATRIC22995515. VBIProMar103344_0943.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAVHRQSWP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPMAR167546:GH1Y-996-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PROM0
AccessionPrimary (citable) accession number: A3PCW8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries