A3PBJ6 (SYE_PROM0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	P9301_04981

Organism

Prochlorococcus marinus (strain MIT 9301) [Complete proteome] [HAMAP]

Taxonomic identifier

167546 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000001933
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3PBJ6 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: D138600DECDBC7A2	FASTA	476	55,323
	10 20 30 40 50 60 MEKRLRLAPS PTGLFHIGTA RTALFNWLYA QKIGGKFLIR IEDTDFLRSK SEYTKNILEG 70 80 90 100 110 120 LKWLGLKWDE EPIKQSDRIS IHKSYIKKLL ECGAAYRCFT SEDEISELRE EQKKKGLPPK 130 140 150 160 170 180 HDNRHRSLSK EEIETFISQG RTSVIRFKID EKIEIKWIDQ IRGEIKWQGK DLGGDLVLSR 190 200 210 220 230 240 RAKGYEIGDP LYNLAVVVDD NFMNITHVVR GEDHISNTAK QILIYKALNF NLPTFSHTPL 250 260 270 280 290 300 ILNSEGKKLS KRDCVTSIDE FREMGYLPEA LSNYMAFLGW SPKSADREIL SLEEISKIFD 310 320 330 340 350 360 LSEINKAGAK FSWEKLNWIN SQYIKNIESI KLIEIMRKYW DDNGWKPPSE EWANKLAILI 370 380 390 400 410 420 RDSMTLLKDS IDQSKPFFLI PTIQKEGQDF LEIRESKLSL KLILNYLIEK NTIKLNKEKA 430 440 450 460 470 KEIINEISKK HNIKKGILMK SLRVAFFGSL SGPDLIQSWE LFAESKTDRT RIERCL

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000576 Genomic DNA. Translation: ABO17121.1.
RefSeq	YP_001090722.1. NC_009091.1.
3D structure databases
ProteinModelPortal	A3PBJ6.
ModBase	Search...
Protein-protein interaction databases
STRING	A3PBJ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4912505.
GenomeReviews	Gene locus P9301_04981 in contig CP000576_GR.
KEGG	pmg:P9301_04981.
PATRIC	22994587. VBIProMar103344_0485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
PhylomeDB	A3PBJ6.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PMAR167546:P9301ORF_0509-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PROM0

Accession

Primary (citable) accession number: A3PBJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program