A3PAY5 (ISPH_PROM0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	P9301_02871

Organism

Prochlorococcus marinus (strain MIT 9301) [Complete proteome] [HAMAP]

Taxonomic identifier

167546 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021150

Sequences

	Sequence		Length	Mass (Da)
	A3PAY5 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 4ADCCC3CE344D166	FASTA	398	44,932
	10 20 30 40 50 60 MDTQAFRRSL HHSDRYNRRG FDSPTKRAQA LEEAYQSDLI SSIRDNGFTY TKGRLNIKLA 70 80 90 100 110 120 QAFGFCWGVE RAVAMAYETR RHYPNENIWI TNEIIHNPSV NDHLRKMNVK FISAKNGIKD 130 140 150 160 170 180 FSLVSNGDVV ILPAFGATVQ EMKLLHEKGC HIIDTTCPWV SKVWHTVEKH KKHVFTSIIH 190 200 210 220 230 240 GKFKHEETLA TSSFAGKYLV VLDLEEANYV SEYILGRGNR NEFMNKFAKA CSNGFDPDKD 250 260 270 280 290 300 LERVGVANQT TMLKSETEEI GKVFERTMLK KFGPENLNSH FLAFNTICDA TEERQDAMFS 310 320 330 340 350 360 LVDEDLDILV VIGGFNSSNT THLQEIAITK NISSFHIDTP ERISVKENSI FHKPLGSELE 370 380 390 LKNNFLPSGK INVGITSGAS TPDKVVADVI EKLIDIAS

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000576 Genomic DNA. Translation: ABO16910.1.
RefSeq	YP_001090511.1. NC_009091.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3PAY5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4911153.
GenomeReviews	Gene locus P9301_02871 in contig CP000576_GR.
KEGG	pmg:P9301_02871.
PATRIC	22994165. VBIProMar103344_0284.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG286221.
OMA	VQEMQLL.
PhylomeDB	A3PAY5.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	PMAR167546:P9301ORF_0291-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_PROM0

Accession

Primary (citable) accession number: A3PAY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program