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A3PAV8

- SYI_PROM0

UniProt

A3PAV8 - SYI_PROM0

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9301)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
Binding sitei626 – 6261ATPUniRule annotation
Metal bindingi936 – 9361ZincUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation
Metal bindingi956 – 9561ZincUniRule annotation
Metal bindingi959 – 9591ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR167546:GH1Y-265-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:P9301_02601
OrganismiProchlorococcus marinus (strain MIT 9301)
Taxonomic identifieri167546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001430: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligasePRO_1000022097Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi167546.P9301_02601.

Structurei

3D structure databases

ProteinModelPortaliA3PAV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionAdd
BLAST
Motifi623 – 6275"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEVMDEIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3PAV8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSQNSKEQK SDFSYKETLN LLKTDFSMRA NSVLREPEIQ NFWANNDIDF
60 70 80 90 100
QLGSSNSGEI FTLHDGPPYA NGALHMGHAL NKVLKDIINK YKTLKGFRVH
110 120 130 140 150
FVPGWDCHGL PIELKVLQNL KSDERKNLDT LNLRKKATDY AHIQINNQKE
160 170 180 190 200
GFKRWGIWGD WNNPYLTLKK SYESAQIGVF GKMFLNGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPDDHYSK SIYVSLKITK IPEEIQLNFI QKNINIKKDF
260 270 280 290 300
FQNNSFITIW TTTPWTIPAN EAVAVNPKIN YIFAIDEEKR IYLFAKDLCS
310 320 330 340 350
EISKKFNKDF KVLLEVKGSK LENIEYQHPS KNKNCRIVIG GDYITTESGT
360 370 380 390 400
GIVHTAPGHG IDDFNVGQKY DLPITCVVDE KGNLNEYSGQ FKGSNVLKDA
410 420 430 440 450
NDLIIEYLKV NNLLLLQENY KHRYPYDWRT KKPTIFRATE QWFASVNGFR
460 470 480 490 500
SSALKAIEDV EWMPETGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYKKN
510 520 530 540 550
GNEILLNKEI INHIQELFSE HGADIWWDWD VKNLLPENYA KESDLWKKGK
560 570 580 590 600
DTMDVWFDSG SSWAAVCELR SELKYPADLY LEGSDQHRGW FQSSLLTSVA
610 620 630 640 650
VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GNNKKTDPAY
660 670 680 690 700
GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD
710 720 730 740 750
PKIDSFEIDQ LPLLDQWMLG RLVEVTDQIS NAYENYEFSK FFQILQSFCV
760 770 780 790 800
VDLSNFYLDI AKDRLYVSSK SQFRRRSCQF VMSKVVENLA VLISPVLCHM
810 820 830 840 850
AEDIWQNIPY STKEKSVFQR GWPIFSQSWK NKILNEHISN LRNLRVEINK
860 870 880 890 900
AIEGCRNKQI IGAALETEVN YLPEDKALKD SLTWLKEFGN QDVDLFRDWL
910 920 930 940 950
IVSNFQVVSD LVDNSLATDN NALGKIQINK AQGQKCDRCW HYQKETFNGI
960
QNTKLCKRCS NIINFEFI
Length:968
Mass (Da):111,766
Last modified:April 3, 2007 - v1
Checksum:i84C0F9ECCF6149ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000576 Genomic DNA. Translation: ABO16883.1.
RefSeqiWP_011862281.1. NC_009091.1.
YP_001090484.1. NC_009091.1.

Genome annotation databases

EnsemblBacteriaiABO16883; ABO16883; P9301_02601.
GeneIDi4910987.
KEGGipmg:P9301_02601.
PATRICi22994109. VBIProMar103344_0257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000576 Genomic DNA. Translation: ABO16883.1 .
RefSeqi WP_011862281.1. NC_009091.1.
YP_001090484.1. NC_009091.1.

3D structure databases

ProteinModelPortali A3PAV8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167546.P9301_02601.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO16883 ; ABO16883 ; P9301_02601 .
GeneIDi 4910987.
KEGGi pmg:P9301_02601.
PATRICi 22994109. VBIProMar103344_0257.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EVMDEIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR167546:GH1Y-265-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9301.

Entry informationi

Entry nameiSYI_PROM0
AccessioniPrimary (citable) accession number: A3PAV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: November 26, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3