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A3PAV8

- SYI_PROM0

UniProt

A3PAV8 - SYI_PROM0

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9301)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
    Binding sitei626 – 6261ATPUniRule annotation
    Metal bindingi936 – 9361ZincUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation
    Metal bindingi956 – 9561ZincUniRule annotation
    Metal bindingi959 – 9591ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPMAR167546:GH1Y-265-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:P9301_02601
    OrganismiProchlorococcus marinus (strain MIT 9301)
    Taxonomic identifieri167546 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
    ProteomesiUP000001430: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 968968Isoleucine--tRNA ligasePRO_1000022097Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi167546.P9301_02601.

    Structurei

    3D structure databases

    ProteinModelPortaliA3PAV8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 7811"HIGH" regionAdd
    BLAST
    Motifi623 – 6275"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiEVMDEIT.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3PAV8-1 [UniParc]FASTAAdd to Basket

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    MKSQNSKEQK SDFSYKETLN LLKTDFSMRA NSVLREPEIQ NFWANNDIDF    50
    QLGSSNSGEI FTLHDGPPYA NGALHMGHAL NKVLKDIINK YKTLKGFRVH 100
    FVPGWDCHGL PIELKVLQNL KSDERKNLDT LNLRKKATDY AHIQINNQKE 150
    GFKRWGIWGD WNNPYLTLKK SYESAQIGVF GKMFLNGYIY RGLKPVHWSP 200
    SSRTALAEAE LEYPDDHYSK SIYVSLKITK IPEEIQLNFI QKNINIKKDF 250
    FQNNSFITIW TTTPWTIPAN EAVAVNPKIN YIFAIDEEKR IYLFAKDLCS 300
    EISKKFNKDF KVLLEVKGSK LENIEYQHPS KNKNCRIVIG GDYITTESGT 350
    GIVHTAPGHG IDDFNVGQKY DLPITCVVDE KGNLNEYSGQ FKGSNVLKDA 400
    NDLIIEYLKV NNLLLLQENY KHRYPYDWRT KKPTIFRATE QWFASVNGFR 450
    SSALKAIEDV EWMPETGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYKKN 500
    GNEILLNKEI INHIQELFSE HGADIWWDWD VKNLLPENYA KESDLWKKGK 550
    DTMDVWFDSG SSWAAVCELR SELKYPADLY LEGSDQHRGW FQSSLLTSVA 600
    VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GNNKKTDPAY 650
    GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD 700
    PKIDSFEIDQ LPLLDQWMLG RLVEVTDQIS NAYENYEFSK FFQILQSFCV 750
    VDLSNFYLDI AKDRLYVSSK SQFRRRSCQF VMSKVVENLA VLISPVLCHM 800
    AEDIWQNIPY STKEKSVFQR GWPIFSQSWK NKILNEHISN LRNLRVEINK 850
    AIEGCRNKQI IGAALETEVN YLPEDKALKD SLTWLKEFGN QDVDLFRDWL 900
    IVSNFQVVSD LVDNSLATDN NALGKIQINK AQGQKCDRCW HYQKETFNGI 950
    QNTKLCKRCS NIINFEFI 968
    Length:968
    Mass (Da):111,766
    Last modified:April 3, 2007 - v1
    Checksum:i84C0F9ECCF6149ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000576 Genomic DNA. Translation: ABO16883.1.
    RefSeqiYP_001090484.1. NC_009091.1.

    Genome annotation databases

    EnsemblBacteriaiABO16883; ABO16883; P9301_02601.
    GeneIDi4910987.
    KEGGipmg:P9301_02601.
    PATRICi22994109. VBIProMar103344_0257.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000576 Genomic DNA. Translation: ABO16883.1 .
    RefSeqi YP_001090484.1. NC_009091.1.

    3D structure databases

    ProteinModelPortali A3PAV8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 167546.P9301_02601.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO16883 ; ABO16883 ; P9301_02601 .
    GeneIDi 4910987.
    KEGGi pmg:P9301_02601.
    PATRICi 22994109. VBIProMar103344_0257.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi EVMDEIT.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PMAR167546:GH1Y-265-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MIT 9301.

    Entry informationi

    Entry nameiSYI_PROM0
    AccessioniPrimary (citable) accession number: A3PAV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3