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A3PAV8

- SYI_PROM0

UniProt

A3PAV8 - SYI_PROM0

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Protein
Isoleucine--tRNA ligase
Gene
ileS, P9301_02601
Organism
Prochlorococcus marinus (strain MIT 9301)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylate By similarity
Binding sitei626 – 6261ATP By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity
Metal bindingi956 – 9561Zinc By similarity
Metal bindingi959 – 9591Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR167546:GH1Y-265-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:P9301_02601
OrganismiProchlorococcus marinus (strain MIT 9301)
Taxonomic identifieri167546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001430: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022097Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi167546.P9301_02601.

Structurei

3D structure databases

ProteinModelPortaliA3PAV8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotation
Add
BLAST
Motifi623 – 6275"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEVMDEIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3PAV8-1 [UniParc]FASTAAdd to Basket

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MKSQNSKEQK SDFSYKETLN LLKTDFSMRA NSVLREPEIQ NFWANNDIDF    50
QLGSSNSGEI FTLHDGPPYA NGALHMGHAL NKVLKDIINK YKTLKGFRVH 100
FVPGWDCHGL PIELKVLQNL KSDERKNLDT LNLRKKATDY AHIQINNQKE 150
GFKRWGIWGD WNNPYLTLKK SYESAQIGVF GKMFLNGYIY RGLKPVHWSP 200
SSRTALAEAE LEYPDDHYSK SIYVSLKITK IPEEIQLNFI QKNINIKKDF 250
FQNNSFITIW TTTPWTIPAN EAVAVNPKIN YIFAIDEEKR IYLFAKDLCS 300
EISKKFNKDF KVLLEVKGSK LENIEYQHPS KNKNCRIVIG GDYITTESGT 350
GIVHTAPGHG IDDFNVGQKY DLPITCVVDE KGNLNEYSGQ FKGSNVLKDA 400
NDLIIEYLKV NNLLLLQENY KHRYPYDWRT KKPTIFRATE QWFASVNGFR 450
SSALKAIEDV EWMPETGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYKKN 500
GNEILLNKEI INHIQELFSE HGADIWWDWD VKNLLPENYA KESDLWKKGK 550
DTMDVWFDSG SSWAAVCELR SELKYPADLY LEGSDQHRGW FQSSLLTSVA 600
VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GNNKKTDPAY 650
GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD 700
PKIDSFEIDQ LPLLDQWMLG RLVEVTDQIS NAYENYEFSK FFQILQSFCV 750
VDLSNFYLDI AKDRLYVSSK SQFRRRSCQF VMSKVVENLA VLISPVLCHM 800
AEDIWQNIPY STKEKSVFQR GWPIFSQSWK NKILNEHISN LRNLRVEINK 850
AIEGCRNKQI IGAALETEVN YLPEDKALKD SLTWLKEFGN QDVDLFRDWL 900
IVSNFQVVSD LVDNSLATDN NALGKIQINK AQGQKCDRCW HYQKETFNGI 950
QNTKLCKRCS NIINFEFI 968
Length:968
Mass (Da):111,766
Last modified:April 3, 2007 - v1
Checksum:i84C0F9ECCF6149ED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000576 Genomic DNA. Translation: ABO16883.1.
RefSeqiYP_001090484.1. NC_009091.1.

Genome annotation databases

EnsemblBacteriaiABO16883; ABO16883; P9301_02601.
GeneIDi4910987.
KEGGipmg:P9301_02601.
PATRICi22994109. VBIProMar103344_0257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000576 Genomic DNA. Translation: ABO16883.1 .
RefSeqi YP_001090484.1. NC_009091.1.

3D structure databases

ProteinModelPortali A3PAV8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 167546.P9301_02601.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO16883 ; ABO16883 ; P9301_02601 .
GeneIDi 4910987.
KEGGi pmg:P9301_02601.
PATRICi 22994109. VBIProMar103344_0257.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EVMDEIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR167546:GH1Y-265-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9301.

Entry informationi

Entry nameiSYI_PROM0
AccessioniPrimary (citable) accession number: A3PAV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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