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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Burkholderia pseudomallei (strain 1106a)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126ATPUniRule annotation1
Binding sitei172ATPUniRule annotation1
Binding sitei199Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:BURPS1106A_A1518
OrganismiBurkholderia pseudomallei (strain 1106a)
Taxonomic identifieri357348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000006738 Componenti: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838271 – 276Hydroxyethylthiazole kinaseAdd BLAST276

Proteomic databases

PRIDEiA3P5E2

Structurei

3D structure databases

ProteinModelPortaliA3P5E2
SMRiA3P5E2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352
KOiK00878

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit

Sequencei

Sequence statusi: Complete.

A3P5E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESISWNTPS VRDALAAVKR DAPFVYGLTN YVAANLSANV LLAVGAAPAI
60 70 80 90 100
GAAADWPARF GAGANALWIN TAALMSSGAD TLLTAARAAS KAGTRWVLDP
110 120 130 140 150
VALGAGAPEY DAIVRDLLAL RPTVIRGNAS ELIALAGGTA AGKGVDTTAS
160 170 180 190 200
PESALAFIGD LARRSGAVVA VSGPTDYVTD GVATLAVAGG DARLTRVTGA
210 220 230 240 250
GCALGALIAA LLAQRGAALA AASAAHAIYA TAAERAADAR GTASFAVRFV
260 270
DELSLLDPAE SSRDRSAGQI GAKRRE
Length:276
Mass (Da):27,510
Last modified:September 22, 2009 - v2
Checksum:i1F7B2E4B381E6D6E
GO

Sequence cautioni

The sequence ABN95350 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000573 Genomic DNA Translation: ABN95350.1 Different initiation.
RefSeqiWP_004542754.1, NC_009078.1

Genome annotation databases

EnsemblBacteriaiABN95350; ABN95350; BURPS1106A_A1518
KEGGibpl:BURPS1106A_A1518

Similar proteinsi

Entry informationi

Entry nameiTHIM_BURP0
AccessioniPrimary (citable) accession number: A3P5E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: May 23, 2018
This is version 64 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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