Reviewed, UniProtKB/Swiss-Prot A3NZL7 (MURD_BURP0)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BURPS1106A_3552

Organism

Burkholderia pseudomallei (strain 1106a) [Complete proteome] [HAMAP]

Taxonomic identifier

357348 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000301419
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A3NZL7-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 5F5BD80F4EB8C5D7	FASTA	504	52,549
	10 20 30 40 50 60 MFGDRQRPMV LVLGLGESGL AIARWCARHG CRLRVADTRE TPPNLAALTA AGVDFEFVGG 70 80 90 100 110 120 AFSPALVDGG IELVALSPGL SPLAEDLAPL VAAARERGIP VWGELEFFAQ ALAALGANGY 130 140 150 160 170 180 APKVIAITGT NGKTTTTSLA GLLCERAGKK VAVAGNISPA MLDKLTEAID AAALPDVWVL 190 200 210 220 230 240 ELSSFQLDTA HTFAPDAATI LNITQDHLDW HGGFAAYAAA KGRVFGPRTV RVLNRDDAEV 250 260 270 280 290 300 MRFAPPAAAA DAPRAVTFGL NEPAADGDYG LLRENGIAWL VEAIDRDGAD APAAPSRRRK 310 320 330 340 350 360 QEAANPPDIA LKRLMPADAL RIRGLHNAAN ALAAYALARA IGLPAAPLLH GLREYRGEPH 370 380 390 400 410 420 RVEVIATLDG VDYVDDSKGT NVGATVAALD GLAQRAVLIA GGDGKGQDFE PLAAPVARWC 430 440 450 460 470 480 RAVMLIGRDA PALREALADT GVPLADHATL EAAVRAASAL AQPGDAVLLS PACASLDMFR 490 500 NYAHRADVFR SAVEDIALEK GTTL

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000572 Genomic DNA. Translation: ABN90880.1.
RefSeq	YP_001067788.1.
3D structure databases
SMR	A3NZL7. Positions 5-496.
ModBase	Search...
Protein-protein interaction databases
STRING	A3NZL7.
Genome annotation databases
GeneID	4899618.
GenomeReviews	Gene locus BURPS1106A_3552 in contig CP000572_GR.
KEGG	bpl:BURPS1106A_3552.
TIGR	BURPS1106A_3552.
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	VKYYNDS.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BURP0

Accession

Primary (citable) accession number: A3NZL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)