Reviewed, UniProtKB/Swiss-Prot A3NZ64 (PUR9_BURP0)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	BURPS1106A_3398

Organism

Burkholderia pseudomallei (strain 1106a) [Complete proteome] [HAMAP]

Taxonomic identifier

357348 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000018858

Sequences

	Sequence		Length	Mass (Da)
	A3NZ64-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: CDDA7B76B25BB0E6	FASTA	521	55,458
	10 20 30 40 50 60 MIKQALISVS DKTGIVDFAK ALSALGVKLL STGGTAKLLA DAGLPVTEVA DYTGFPEMLD 70 80 90 100 110 120 GRVKTLHPKV HGGILARRDL PEHMQALEAH GIPTIDLLVV NLYPFVQTIA KDDCTLADAI 130 140 150 160 170 180 ENIDIGGPTM LRSAAKNHRD VTVVVDPADY AVVLDEMKAN GNTLGYKTNF RLATKVFAHT 190 200 210 220 230 240 AQYDGAITNY LTSLGDDLQH GSRSAYPATL NLAFDKVQDL RYGENPHQSA AFYRDVATPA 250 260 270 280 290 300 GALANYRQLQ GKELSYNNIA DSDAAWECVK TFDAPACVII KHANPCGVAV GADAGEAYAK 310 320 330 340 350 360 AFQTDPTSAF GGIIAFNREV DEAAAQAVAK QFVEVLIAPS FSDAAKQVFA AKQNVRLLEI 370 380 390 400 410 420 ALGEGHNAFD LKRVGGGLLV QSLDSKNVQP RELRVVTKRH PTPKEMDDLL FAWRVAKYVK 430 440 450 460 470 480 SNAIVFCGNG MTLGVGAGQM SRVDSARIAS IKAQNAGLTL AGSAVASDAF FPFRDGLDVV 490 500 510 520 VAAGATCVIQ PGGSVRDDEV IAAADEHNIA MVVTGVRHFR H

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000572 Genomic DNA. Translation: ABN89209.1.
RefSeq	YP_001067635.1.
3D structure databases
SMR	A3NZ64. Positions 2-521.
ModBase	Search...
Protein-protein interaction databases
STRING	A3NZ64.
Genome annotation databases
GeneID	4901541.
GenomeReviews	Gene locus BURPS1106A_3398 in contig CP000572_GR.
KEGG	bpl:BURPS1106A_3398.
TIGR	BURPS1106A_3398.
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HBG498048.
OMA	VVKHVKS.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BURP0

Accession

Primary (citable) accession number: A3NZ64

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)