Reviewed, UniProtKB/Swiss-Prot A3NYY2 (HEMH_BURP0)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	BURPS1106A_3316

Organism

Burkholderia pseudomallei (strain 1106a) [Complete proteome] [HAMAP]

Taxonomic identifier

357348 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Ferrochelatase HAMAP MF_00323		PRO_1000019282
Sites
	Metal binding	226	1	Iron By similarity
	Metal binding	307	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3NYY2-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: CB37193FB9181959	FASTA	367	40,571
	10 20 30 40 50 60 MSFDSVPRHA LSMRFDLEPP SHASAAHRVA VLLVNLGTPD APTPRAVRRY LAQFLSDPRV 70 80 90 100 110 120 VEIPQLVWQV ILRTLILPLR GRASAKKYAA VWLPEGSPLR VYTERQVESV KPLFAANGYR 130 140 150 160 170 180 VIVDYAMRYG TPSIADVLAQ LKRAGAERVL LLPMYPQYSS STTATAFDAA FAALGRMRNQ 190 200 210 220 230 240 PEVRTVRHYA DHPAYIHALA EQVRQYWAAH GRPAFDAGDK LVLSFHGVPK RTLDLGDPYH 250 260 270 280 290 300 DQCQQTAALL MSALGLTTFE CRVTFQSRFG KAEWLQPYTA PTLKELGAAG VRRADVFCPG 310 320 330 340 350 360 FTADCLETIE EIGIEVRDEF VHGGGKEFHR IPCLNASPAW IAALGEIAAE NLQGWPVRVA MAPEAVS

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000572 Genomic DNA. Translation: ABN91419.1.
RefSeq	YP_001067553.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3NYY2.
Genome annotation databases
GeneID	4902784.
GenomeReviews	Gene locus BURPS1106A_3316 in contig CP000572_GR.
KEGG	bpl:BURPS1106A_3316.
TIGR	BURPS1106A_3316.
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	TIEEIGM.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURP0

Accession

Primary (citable) accession number: A3NYY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)