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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Burkholderia pseudomallei (strain 1106a)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei355SubstrateUniRule annotation1
Active sitei356Proton donor/acceptorUniRule annotation1
Active sitei486UniRule annotation1
Binding sitei487SubstrateUniRule annotation1
Sitei499Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotationImported
Ordered Locus Names:BURPS1106A_2886Imported
OrganismiBurkholderia pseudomallei (strain 1106a)Imported
Taxonomic identifieri357348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000006738 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA3NXR0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 510Lyase_1InterPro annotationAdd BLAST331
Domaini576 – 629FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 268Substrate bindingUniRule annotation3
Regioni297 – 300Substrate binding (B site)UniRule annotation4
Regioni307 – 309Substrate bindingUniRule annotation3
Regioni492 – 494Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A3NXR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVRAIAHA RRKSVSDHSF LPKGVERSMT TVDSPRDANG PPRFAASGFA
60 70 80 90 100
TPRRPDDTDD TGDADETAAT VPHRTRALTT PPRRKTIAAP RIDARTRRDR
110 120 130 140 150
PARRATHARR ALADFRAPAP PIARGTTRNR ARRYACPSVD AVARTAPSPH
160 170 180 190 200
AHRAAPARRP PSSEGAIMSE AVRMERDTFG EIAVPAARLW GAQTQRSLQN
210 220 230 240 250
FKISTEKQSP ELIHALALIK RAAAAVNLEL GVLAQDKANA IVAAADEIIA
260 270 280 290 300
GRHADEFPLA VWQTGSGTQT NMNLNEVIAN RASELLGGER GESRAVHPND
310 320 330 340 350
DVNRGQSSND VFPTAMHIAA ARAIVDHLLP ALRTLRATLD AKAAAFADIV
360 370 380 390 400
KIGRTHLQDA TPLTLGQEFS GYVAQLDQGI RHVEAALPHL YELAQGGTAV
410 420 430 440 450
GTGLNAHPKF AAGVAAEIGR LTGLPFVSAP NKFEVMAAAD ALVFAHGALK
460 470 480 490 500
TVAASLMKIA NDIRWLASGP RCGLGELSIP ENEPGSSIMP GKVNPTQSEA
510 520 530 540 550
VTMLCCQVFG NDVAVNFGGA SGNFELNVFR PMIAHNVLQS VRLLADGARG
560 570 580 590 600
FNDHCAVGIE PNRARIDALL NESLMLVTAL NPHIGYDKAA QIAKKAHKEG
610 620 630
TTLKAAALAL GYVTDAQFDE WVRPEHMVGN R
Length:631
Mass (Da):67,355
Last modified:April 3, 2007 - v1
Checksum:iCF572B183EA42FAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000572 Genomic DNA. Translation: ABN91344.1.

Genome annotation databases

EnsemblBacteriaiABN91344; ABN91344; BURPS1106A_2886.
KEGGibpl:BURPS1106A_2886.

Similar proteinsi

Entry informationi

Entry nameiA3NXR0_BURP0
AccessioniPrimary (citable) accession number: A3NXR0
Entry historyiIntegrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: April 3, 2007
Last modified: September 27, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported