A3NUI8 (SYFA_BURP0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BURPS1106A_1739

Organism

Burkholderia pseudomallei (strain 1106a) [Complete proteome] [HAMAP]

Taxonomic identifier

357348 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000006806
Sites
	Metal binding	258	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3NUI8 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: A7D14A8D41CC8576	FASTA	337	38,024
	10 20 30 40 50 60 MDLDQIVADA QQSFEGAADI TTLENEKARF LGKSGALTEL LKGLGKLDPE TRKTEGARIN 70 80 90 100 110 120 VAKQQVEAAL NARRQALADA LLNQRLAAEA IDVTLPGRGA GAGSLHPVMR TWERVEQIFR 130 140 150 160 170 180 SIGFDVADGP EIETDWYNFT ALNSPENHPA RSMQDTFYVD GKDADGRPLL LRTHTSPMQV 190 200 210 220 230 240 RYARMNRPPI KVIAPGRTYR VDSDATHSPM FNQVEGLWID ENVSFADLKG VYTDFLKKFF 250 260 270 280 290 300 ERDDILVRFR PSYFPFTEPS AEIDMMFEHG KNAGKWLEIS GSGQVHPTVI RNMGLDPERY 310 320 330 IGFAFGSGLE RLTMLRYGVQ DLRLFFENDL RFLRQFA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1106a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000572 Genomic DNA. Translation: ABN91142.1.
RefSeq	YP_001066009.3. NC_009076.1.
3D structure databases
ProteinModelPortal	A3NUI8.
ModBase	Search...
Protein-protein interaction databases
STRING	A3NUI8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4902188.
GenomeReviews	Gene locus BURPS1106A_1739 in contig CP000572_GR.
KEGG	bpl:BURPS1106A_1739.
PATRIC	19221995. VBIBurPse14980_1670.
TIGR	BURPS1106A_1739.
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BURP0

Accession

Primary (citable) accession number: A3NUI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program