Reviewed, UniProtKB/Swiss-Prot A3NC70 (METE_BURP6)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BURPS668_2923

Organism

Burkholderia pseudomallei (strain 668) [Complete proteome] [HAMAP]

Taxonomic identifier

320373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000071606
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3NC70-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 08A4E75BB2873364	FASTA	764	84,391
	10 20 30 40 50 60 MTTAHILGFP RIGAQRELKF ALERYWRDGA SADAERALVD TGRALRAEHW RIERDAGLDC 70 80 90 100 110 120 VTVGDFAWYD HVLTTLAHVG GLPRRFGFDA HALTLADYFA AARGNAAQPA MEMTKWFDTN 130 140 150 160 170 180 YHYLVPEYSP ATTFGPGVEW LFDEVREARA LGHRAKAALV GPLTLLWLGK ARDGLVERLA 190 200 210 220 230 240 LLPRLVPAYR ALLARLREAG VDWVQIDEPI FSLDLPDAWR DAARPTYEAL APGAPKLLVA 250 260 270 280 290 300 TYFDDASEHA ALLKALPVAG LHIDLVRADA QLDAFVADYP ADKVLSCGIV DGRNVWRNDL 310 320 330 340 350 360 DRSLARLAPV RDALGERLWV ATSCSLLHVP VDLAHEPRLD EELKTWLAFA AQKTREVAAL 370 380 390 400 410 420 RDALVKGRAA VAAEFDDAAA AAAARRTSAR IHNPLVKRRV AALTDADARR ASTYSVRAAA 430 440 450 460 470 480 QRARFGLPLL PTTTIGSFPQ TPEIRRARAA FKQGVLDHLG YLEAMREQVR IAIDKQLAYG 490 500 510 520 530 540 LDVLVHGEAE RNDMVEYFGE LLWGFAITSN GWVQSYGSRC VKPPLVYGDV YLPEPMTVGW 550 560 570 580 590 600 ASYAQSLSAK PVKGMLTGPV TMLQWSFVRD DQPRATTALQ IALALRQETL DLEKAGIGMI 610 620 630 640 650 660 QIDEPALREG LPLKARERAA YLDWAVRAFG IAASGVADDT QIHTHMCYSE FGDILPSIAA 670 680 690 700 710 720 LDADVISIET TRSNMELLDA FETFDYPNEI GPGVYDIHSP RVPDADEIER LILLALERIP 730 740 750 760 AQRLWVNPDC GLKTREWRQV DAALAAMVDA AKRVRQKVEE AVPA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000570 Genomic DNA. Translation: ABN84840.1.
RefSeq	YP_001059941.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3NC70.
Genome annotation databases
GeneID	4883667.
GenomeReviews	Gene locus BURPS668_2923 in contig CP000570_GR.
KEGG	bpd:BURPS668_2923.
NMPDR	fig|320373.3.peg.4435.
TIGR	BURPS668_2923.
Phylogenomic databases
OMA	TYLWLSK.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURP6

Accession

Primary (citable) accession number: A3NC70

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)