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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Burkholderia pseudomallei (strain 668)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei417 – 4171NucleophileUniRule annotation
Active sitei472 – 4721Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciBPSE320373:GJ9C-1540-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:BURPS668_1543
OrganismiBurkholderia pseudomallei (strain 668)
Taxonomic identifieri320373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000002153: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7387381,4-alpha-glucan branching enzyme GlgBPRO_1000044972Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi320373.BURPS668_1543.

Structurei

3D structure databases

ProteinModelPortaliA3N8B3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A3N8B3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDAPFDRAD IDALLGARHP DPFACLGPHR VGDATVVRTL LPGALRVRAI
60 70 80 90 100
AAGGGVLGEL RQVDPAGCFA GALPDGRERG ERPRYRLSID WPDARQDVED
110 120 130 140 150
AYAFGTLLDE DALARFAAGD PRAALACLGA RALDMDGVPG VRFAVWAPGA
160 170 180 190 200
SRVSVVGDFN GWDARRHPMR LRRPWGVWEL FVPRIGAGER YKFALRARDG
210 220 230 240 250
AALPLKADPC ACRTEAPPRT ASIVADLDAL ERFGWHDDAW LRARASLDLA
260 270 280 290 300
HAPVSIYEVH PESWLRVAAE GNRSATWDEL AQRLIPYAAG MGFSHVELTP
310 320 330 340 350
IAEYPFGGSW GYQSLSPFAP SARFGPPEGF ARFVEHAHAA GLGVIVDWVP
360 370 380 390 400
AHFPDDPHGL GKFDGTALFE HADPREGWHP DWHTHVFNVG RREVGAFLIA
410 420 430 440 450
SALAWAHRYH VDGIRVDAVA SMLYRDYSRA AGEWVPNVYG GRENLESIAF
460 470 480 490 500
LKHFNDTLHG PAAPPGVATF AEESTAWPGV TAPTAEHGLG FDFKWNMGWM
510 520 530 540 550
HDTLAYLRED PIHRRHHHDR LTFGLVYAFS ERFVLPLSHD EVVHGKGSLA
560 570 580 590 600
AKMPGDAWQR LANLRAYFGF MWAHPGKKLL FMGGEFAQWE EFAHDATPQW
610 620 630 640 650
DLLDAPAHRG VQRLVRDLNR LHAAEPALHA LDDRPAGFAW LVGDDRNNSV
660 670 680 690 700
FAFVRRDDAG RMLVAVCNFT PVPRTDYRLG LPAPGRWAEV LNTDGAAYGG
710 720 730
TDAGNGGAVQ ADEIPAHGER WSAALRLPPL ATLWLRPA
Length:738
Mass (Da):81,116
Last modified:April 3, 2007 - v1
Checksum:i3F9C138A4A088A48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000570 Genomic DNA. Translation: ABN83141.1.
RefSeqiYP_001058584.1. NC_009074.1.

Genome annotation databases

EnsemblBacteriaiABN83141; ABN83141; BURPS668_1543.
GeneIDi4883906.
KEGGibpd:BURPS668_1543.
PATRICi19248982. VBIBurPse82117_1454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000570 Genomic DNA. Translation: ABN83141.1.
RefSeqiYP_001058584.1. NC_009074.1.

3D structure databases

ProteinModelPortaliA3N8B3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi320373.BURPS668_1543.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN83141; ABN83141; BURPS668_1543.
GeneIDi4883906.
KEGGibpd:BURPS668_1543.
PATRICi19248982. VBIBurPse82117_1454.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciBPSE320373:GJ9C-1540-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. DeShazer D., Woods D.E., Nierman W.C.
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 668.

Entry informationi

Entry nameiGLGB_BURP6
AccessioniPrimary (citable) accession number: A3N8B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: February 4, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.