Reviewed, UniProtKB/Swiss-Prot A3N8B3 (GLGB_BURP6)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	BURPS668_1543

Organism

Burkholderia pseudomallei (strain 668) [Complete proteome] [HAMAP]

Taxonomic identifier

320373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000044972
Sites
	Active site	312	1	By similarity
	Active site	347	1	By similarity
	Active site	352	1	By similarity
	Active site	415	1	By similarity
	Active site	417	1	By similarity
	Active site	472	1	By similarity
	Active site	539	1	By similarity
	Active site	540	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N8B3-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 3F9C138A4A088A48	FASTA	738	81,116
	10 20 30 40 50 60 MTDAPFDRAD IDALLGARHP DPFACLGPHR VGDATVVRTL LPGALRVRAI AAGGGVLGEL 70 80 90 100 110 120 RQVDPAGCFA GALPDGRERG ERPRYRLSID WPDARQDVED AYAFGTLLDE DALARFAAGD 130 140 150 160 170 180 PRAALACLGA RALDMDGVPG VRFAVWAPGA SRVSVVGDFN GWDARRHPMR LRRPWGVWEL 190 200 210 220 230 240 FVPRIGAGER YKFALRARDG AALPLKADPC ACRTEAPPRT ASIVADLDAL ERFGWHDDAW 250 260 270 280 290 300 LRARASLDLA HAPVSIYEVH PESWLRVAAE GNRSATWDEL AQRLIPYAAG MGFSHVELTP 310 320 330 340 350 360 IAEYPFGGSW GYQSLSPFAP SARFGPPEGF ARFVEHAHAA GLGVIVDWVP AHFPDDPHGL 370 380 390 400 410 420 GKFDGTALFE HADPREGWHP DWHTHVFNVG RREVGAFLIA SALAWAHRYH VDGIRVDAVA 430 440 450 460 470 480 SMLYRDYSRA AGEWVPNVYG GRENLESIAF LKHFNDTLHG PAAPPGVATF AEESTAWPGV 490 500 510 520 530 540 TAPTAEHGLG FDFKWNMGWM HDTLAYLRED PIHRRHHHDR LTFGLVYAFS ERFVLPLSHD 550 560 570 580 590 600 EVVHGKGSLA AKMPGDAWQR LANLRAYFGF MWAHPGKKLL FMGGEFAQWE EFAHDATPQW 610 620 630 640 650 660 DLLDAPAHRG VQRLVRDLNR LHAAEPALHA LDDRPAGFAW LVGDDRNNSV FAFVRRDDAG 670 680 690 700 710 720 RMLVAVCNFT PVPRTDYRLG LPAPGRWAEV LNTDGAAYGG TDAGNGGAVQ ADEIPAHGER 730 WSAALRLPPL ATLWLRPA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000570 Genomic DNA. Translation: ABN83141.1.
RefSeq	YP_001058584.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3N8B3.
Genome annotation databases
GeneID	4883906.
GenomeReviews	Gene locus BURPS668_1543 in contig CP000570_GR.
KEGG	bpd:BURPS668_1543.
NMPDR	fig|320373.3.peg.3156.
TIGR	BURPS668_1543.
Phylogenomic databases
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURP6

Accession

Primary (citable) accession number: A3N8B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)