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A3N700 (HEM3_BURP6) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Porphobilinogen deaminase

Short name=PBG
EC=2.5.1.61
Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase
Gene names
Name:hemC
Ordered Locus Names:BURPS668_1071
OrganismBurkholderia pseudomallei (strain 668) [Complete proteome] [HAMAP]
Taxonomic identifier320373 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps By similarity. HAMAP-Rule MF_00260

Catalytic activity

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP-Rule MF_00260

Cofactor

Binds 1 dipyrromethane group covalently By similarity. HAMAP-Rule MF_00260

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP-Rule MF_00260

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00260

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group By similarity.

Sequence similarities

Belongs to the HMBS family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpeptidyl-pyrromethane cofactor linkage

Inferred from electronic annotation. Source: InterPro

protoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionhydroxymethylbilane synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Porphobilinogen deaminase HAMAP-Rule MF_00260
PRO_1000047740

Amino acid modifications

Modified residue2501S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
A3N700 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 0867C598860852BD

FASTA32934,437
        10         20         30         40         50         60 
MNSETLPAEL PATLTIASRE SRLAMWQAEH VRDALRKLYP ACDVKILGMT TRGDQILDRT 

        70         80         90        100        110        120 
LSKVGGKGLF VKELESALAD GRADLAVHSL KDVPMVLPEG FALAAVMSRE DPRDAFVSND 

       130        140        150        160        170        180 
YASLDALPAG AVVGTSSLRR EAMLRARHPR LDVRPLRGNL DTRLAKLDRG DYAAIILAAA 

       190        200        210        220        230        240 
GLKRLGLAAR IRALLDVDDS LPAAGQGALG IEIAARRADV AAWLAPLHDH ASALAVEAER 

       250        260        270        280        290        300 
AVSRALGGSC EVPLAAHAVW RGGELHLTGS VSTTDGARVL AAHAHARAAT AADALALGRR 

       310        320 
VSDALERQGA RAIVDALVAA SAQAQKGGA 

« Hide

References

[1]DeShazer D., Woods D.E., Nierman W.C.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 668.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000570 Genomic DNA. Translation: ABN82304.1.
RefSeqYP_001058121.1. NC_009074.1.

3D structure databases

ProteinModelPortalA3N700.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING320373.BURPS668_1071.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN82304; ABN82304; BURPS668_1071.
GeneID4883458.
KEGGbpd:BURPS668_1071.
PATRIC19248097. VBIBurPse82117_1021.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0181.
HOGENOMHOG000228587.
KOK01749.
OMACITERED.
OrthoDBEOG6HB9Z6.
ProtClustDBPRK00072.

Enzyme and pathway databases

BioCycBPSE320373:GJ9C-1070-MONOMER.
UniPathwayUPA00251; UER00319.

Family and domain databases

Gene3D3.30.160.40. 1 hit.
HAMAPMF_00260. Porphobil_deam.
InterProIPR000860. 4pyrrol_synth_OHMeBilane_synth.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERPTHR11557. PTHR11557. 1 hit.
PfamPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSPR00151. PORPHBDMNASE.
SUPFAMSSF54782. SSF54782. 1 hit.
TIGRFAMsTIGR00212. hemC. 1 hit.
PROSITEPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM3_BURP6
AccessionPrimary (citable) accession number: A3N700
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways