A3N700 (HEM3_BURP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	BURPS668_1071

Organism

Burkholderia pseudomallei (strain 668) [Complete proteome] [HAMAP]

Taxonomic identifier

320373 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia pseudomallei

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps By similarity. HAMAP-Rule MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP-Rule MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP-Rule MF_00260
Subunit structure	Monomer By similarity.
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethane group By similarity. HAMAP-Rule MF_00260
Sequence similarities	Belongs to the HMBS family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP peptidyl-pyrromethane cofactor linkage Inferred from electronic annotation. Source: InterPro protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Porphobilinogen deaminase HAMAP-Rule MF_00260		PRO_1000047740
Amino acid modifications
	Modified residue	250	1	S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N700 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 0867C598860852BD	FASTA	329	34,437
	10 20 30 40 50 60 MNSETLPAEL PATLTIASRE SRLAMWQAEH VRDALRKLYP ACDVKILGMT TRGDQILDRT 70 80 90 100 110 120 LSKVGGKGLF VKELESALAD GRADLAVHSL KDVPMVLPEG FALAAVMSRE DPRDAFVSND 130 140 150 160 170 180 YASLDALPAG AVVGTSSLRR EAMLRARHPR LDVRPLRGNL DTRLAKLDRG DYAAIILAAA 190 200 210 220 230 240 GLKRLGLAAR IRALLDVDDS LPAAGQGALG IEIAARRADV AAWLAPLHDH ASALAVEAER 250 260 270 280 290 300 AVSRALGGSC EVPLAAHAVW RGGELHLTGS VSTTDGARVL AAHAHARAAT AADALALGRR 310 320 VSDALERQGA RAIVDALVAA SAQAQKGGA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 668.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000570 Genomic DNA. Translation: ABN82304.1.
RefSeq	YP_001058121.1. NC_009074.1.
3D structure databases
ProteinModelPortal	A3N700.
ModBase	Search...
Protein-protein interaction databases
STRING	320373.BURPS668_1071.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN82304; ABN82304; BURPS668_1071.
GeneID	4883458.
KEGG	bpd:BURPS668_1071.
PATRIC	19248097. VBIBurPse82117_1021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0181.
HOGENOM	HOG000228587.
KO	K01749.
OMA	CLAERAF.
ProtClustDB	PRK00072.
Enzyme and pathway databases
BioCyc	BPSE320373:GJ9C-1070-MONOMER.
UniPathway	UPA00251; UER00319.
Family and domain databases
Gene3D	3.30.160.40. 1 hit.
HAMAP	MF_00260. Porphobil_deam.
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022419. Porphobilin_deaminase_cofac_BS. IPR022417. Porphobilin_deaminase_N. IPR022418. Porphobilinogen_deaminase_C. [Graphical view]
PANTHER	PTHR11557. PTHR11557. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. PF03900. Porphobil_deamC. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
SUPFAM	SSF54782. Porphobil_deam. 1 hit.
TIGRFAMs	TIGR00212. hemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_BURP6

Accession

Primary (citable) accession number: A3N700

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program