A3N6P6 (LSPA_BURP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BURPS668_0966

Organism

Burkholderia pseudomallei (strain 668) [Complete proteome] [HAMAP]

Taxonomic identifier

320373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000038791
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	32 – 52	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Transmembrane	135 – 155	21	Helical; Potential
Sites
	Active site	117	1	By similarity
	Active site	144	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N6P6 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 26B7E87FBD3D2F2C	FASTA	166	18,099
	10 20 30 40 50 60 MAKTLSKSSG GALAPWLGIS LIVILFDQLT KIAVLKTFAY GAMHALTPFF NLTLIYNRGA 70 80 90 100 110 120 AFGFLATAGG WQRWAFTALG IGATLVICYL LKRHGHQRLF SLSLALILGG ALGNVIDRLI 130 140 150 160 YGHVIDFLDF HVGAWHWPAF NLADSAITVG AVLLIYDELR RVRGAR

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 668.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000570 Genomic DNA. Translation: ABN81493.1.
RefSeq	YP_001058017.1. NC_009074.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3N6P6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4883040.
GenomeReviews	Gene locus BURPS668_0966 in contig CP000570_GR.
KEGG	bpd:BURPS668_0966.
PATRIC	19247889. VBIBurPse82117_0918.
TIGR	BURPS668_0966.
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HBG724422.
OMA	NFELRES.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	BPSE320373:BURPS668_0966-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BURP6

Accession

Primary (citable) accession number: A3N6P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program