A3N5U2 (QUEF_BURP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	BURPS668_0661

Organism

Burkholderia pseudomallei (strain 668) [Complete proteome] [HAMAP]

Taxonomic identifier

320373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000062334

Sequences

	Sequence		Length	Mass (Da)
	A3N5U2 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 53ECAF5D759A9272	FASTA	274	30,401
	10 20 30 40 50 60 MNPEHSPLGK ATVYANQYDA SLLFPIPRAG AREQIGIGAP LPFFGTDIWN AYELSWLNAR 70 80 90 100 110 120 GKPQIAIATF YVPAESPNIV ESKSFKLYLG SFAQTAFESA DAVRDALKRD VSAACGASVT 130 140 150 160 170 180 VRLATPAEFR KLQMDELDGL SLDRLDLDAH VYETDPSFLT ASHDEAPVEE TLVTDLLKSN 190 200 210 220 230 240 CPVTGQPDWG SVQIHYVGAP IDHAGLLRYI ISFRNHTGFH EQCVERIFVD ILRACQPVKL 250 260 270 AVYARYTRRG GLDINPFRTN YNQPMPDNAR TARQ

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 668.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000570 Genomic DNA. Translation: ABN83377.1.
RefSeq	YP_001057713.1. NC_009074.1.
3D structure databases
ProteinModelPortal	A3N5U2.
SMR	A3N5U2. Positions 14-274.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N5U2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4883160.
GenomeReviews	Gene locus BURPS668_0661 in contig CP000570_GR.
KEGG	bpd:BURPS668_0661.
PATRIC	19247297. VBIBurPse82117_0623.
TIGR	BURPS668_0661.
Phylogenomic databases
eggNOG	COG2904.
HOGENOM	HBG289139.
OMA	CVERIFC.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	BPSE320373:BURPS668_0661-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BURP6

Accession

Primary (citable) accession number: A3N5U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program