Reviewed, UniProtKB/Swiss-Prot A3N3P3 (PROA_ACTP2)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	APL_1951

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049931

Sequences

	Sequence		Length	Mass (Da)
	A3N3P3-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: AC22649C02FC1CE0	FASTA	412	44,907
	10 20 30 40 50 60 MQMLTLAQQA KIASIELAQF GNVQKNHALL TIAEQLEQRS AEILAANAKD IEFAKNQGIS 70 80 90 100 110 120 TAIIDRLLLN ESRLQGIAND VRNVAKLADP VGQVIDGGVL NSGLKIERQR VPLGVILTIY 130 140 150 160 170 180 EARPNVTIDV ASLCLKTGNA VILRGGKETK FTNAVLVEVV QQALETAGLP KLAVQAVTDP 190 200 210 220 230 240 DRALLLELLK LDRYIDMVIP RGGAGLHQFC KENSTIPVIV GGIGVCHMFV EKSADQEKAL 250 260 270 280 290 300 ELIANAKTQR PSTCNTLETL LVEKAIAGEF LPKLANRMKA LDVTLHTDDL QKTEGIEPLD 310 320 330 340 350 360 DARMRQEWLS LDLNVVVIDN LTKAVEHIRE YGSQHSEAIL TSDYQLARQF VAQVDAAAVY 370 380 390 400 410 INASTRFTDG GEFGLGAEVA VSTQKLHARG PMGLEALTTY KWVCEGDYLV RK

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN75029.1.
RefSeq	YP_001054634.1.
3D structure databases
SMR	A3N3P3. Positions 2-412.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N3P3.
Genome annotation databases
GeneID	4848580.
GenomeReviews	Gene locus APL_1951 in contig CP000569_GR.
KEGG	apl:APL_1951.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	A3N3P3.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACTP2

Accession

Primary (citable) accession number: A3N3P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)