A3N3L9 (TPIS_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	APL_1925

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Triosephosphate isomerase HAMAP MF_00147_B		PRO_0000307420
Sites
	Active site	97	1	Electrophile By similarity
	Active site	169	1	Proton acceptor By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	12	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N3L9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 273D2543F1588216	FASTA	256	26,522
	10 20 30 40 50 60 MARRPLVMGN WKLNGSKAFT KELIAGLKAE LADVKGCDVA IAPPVMYLAE AEAALAGQSV 70 80 90 100 110 120 IALGAQNVDV NVQGAFTGDI STEMLKDFGA KYIIIGHSER RTYHKECDTF IAKKFAALKA 130 140 150 160 170 180 AGLVPVLCIG ETEAENEAGQ TEAVCAKQID AVIDALGVEA FNGAVIAYEP IWAIGTGKSA 190 200 210 220 230 240 TPAQAQAVHA FIRGHIAAKS QAVADQVIIQ YGGSVNDANA AELFTQPDID GALVGGASLK 250 APAFAVIVKA AEKAKA

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN75005.1.
RefSeq	YP_001054610.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3N3L9.
SMR	A3N3L9. Positions 3-255.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N3L9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4848635.
GenomeReviews	Gene locus APL_1925 in contig CP000569_GR.
KEGG	apl:APL_1925.
PATRIC	20748942. VBIActPle94089_2004.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
HOGENOM	HBG708281.
OMA	VCVGETQ.
PhylomeDB	A3N3L9.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	APLE416269:APL_1925-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ACTP2

Accession

Primary (citable) accession number: A3N3L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program