Reviewed, UniProtKB/Swiss-Prot A3N3E9 (ILVC_ACTP2)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	APL_1853

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Ketol-acid reductoisomerase		PRO_1000050473
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A3N3E9-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: C3C0305A17739780	FASTA	493	54,393
	10 20 30 40 50 60 MANYFNTLNL RQKLDQLGRC RFMDRNEFAD GCNFLKGKKI VIVGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LDISYALRAE AIAEKRASFT RATENGFKVG TYQELIPTAD LVINLTPDKQ HSKVVADVVP 130 140 150 160 170 180 LMKQGSAFGY SHGFNIVEEG EQIRKDITVV MTAPKCPGTE VREEYKRGFG VPTLIAVHPE 190 200 210 220 230 240 NDPKGEGMAI AKAWASATGG DRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSIVCYDKL 250 260 270 280 290 300 VADGKDPAYA SKLIQYGWET ITEALKQGGI TLMMDRLSNA AKIRAFELSE EIKVQLNDLY 310 320 330 340 350 360 LKHMDDIISG EFSSTMMADW ANGDVNLFKW REETGKTAFE NSPKADGIKI SEQEYFDNGV 370 380 390 400 410 420 VMVAMVKAGV EMAFDAMVAS GIYEESAYYE SLHELPLIAN TIARKRLYEM NVVISDTAEY 430 440 450 460 470 480 GNYLFSHVAT PILAEKLIPM LQKGDLGEPT PAAEIDNVYL RDINDAIRNH PVELIGQELR 490 GYMTDMKRIS SQG

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74935.1.
RefSeq	YP_001054540.1.
3D structure databases
SMR	A3N3E9. Positions 3-490.
ModBase	Search...
Genome annotation databases
GeneID	4849756.
GenomeReviews	Gene locus APL_1853 in contig CP000569_GR.
KEGG	apl:APL_1853.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ACTP2

Accession

Primary (citable) accession number: A3N3E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)