A3N3A1 (SYGB_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	APL_1803

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › Actinobacillus pleuropneumoniae serovar 5

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255		PRO_1000006348

Sequences

	Sequence		Length	Mass (Da)
	A3N3A1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: C6B06294DC9D8346	FASTA	689	76,379
	10 20 30 40 50 60 MTTQNFLAEI GTEELPPKAL KKLATAFAEN VENELNQADL SFEKVEWFAA PRRLAVKALG 70 80 90 100 110 120 LATAQPSKEI EKRGPAVSAA FDADGKPTKA AEGWARGCGI TVDQADRIAT DKGEWLVHRA 130 140 150 160 170 180 VIEGQPTKNL LVDIISRSLA NLPIPKMMRW GDKTEQFVRP VHTVTLFFGS ELIEGEILGV 190 200 210 220 230 240 KINNVIRGHR FLGEREFTIS HADEYLTALR EKGMVVADFN ERKALILAKS QEKATALGGV 250 260 270 280 290 300 ADIEEDLLDE VTSLVEFPNV LTAKFEERFL AVPAEALVYT MKGDQKYFPI YDKDGKLLPH 310 320 330 340 350 360 FIFVSNINPE DPTAIIEGNE KVVRPRLTDA EFFFKTDLKQ RLEDRLPRLE TVLFQQQLGT 370 380 390 400 410 420 LRDKTARIEA LAGEIAAQIG ADKAKAERAG LLSKCDLMTN MVFEFTDTQG VMGMHYARHD 430 440 450 460 470 480 GEDEEVAVAL NEQYMPRFAG DELPKSLVAC SVALADKFDT LTGIFGIGQA PKGSADPFAL 490 500 510 520 530 540 RRAALGSLRI IVEKNLPLDL EDLVRKSAAL FGNKLTNANV VEDVVDFMLG RFRAWYQDEG 550 560 570 580 590 600 IAVDVIQAVL ARRPTKPADF DARVRAVSHF RTLDSAEALA AANKRVSNIL AKVEGEISIE 610 620 630 640 650 660 IDRTLLVEAE EKALAEQVIS LQTELAPTFA NGEYQTALDR LASLRETVDN FFEKVMVNAE 670 680 DANLRRNRQA ILNNLRNLFL QVADISVLQ

References

[1]

"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN74887.1.
RefSeq	YP_001054492.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3N3A1.
ModBase	Search...
Protein-protein interaction databases
STRING	416269.APL_1803.
Proteomic databases
PRIDE	A3N3A1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN74887; ABN74887; APL_1803.
GeneID	4848617.
KEGG	apl:APL_1803.
PATRIC	20748667. VBIActPle94089_1874.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0751.
HOGENOM	HOG000264302.
KO	K01879.
OMA	GMHYARL.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	APLE416269:GHV7-1872-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta.
InterPro	IPR008909. DALR_anticod-bd. IPR015944. Gly-tRNA-synth_bsu. IPR006194. Gly-tRNA-synth_heterodimer. [Graphical view]
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00211. glyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_ACTP2

Accession

Primary (citable) accession number: A3N3A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program