Reviewed, UniProtKB/Swiss-Prot A3N386 (TRMD_ACTP2)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	APL_1788

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006443
Regions
	Region	137 – 142	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	117	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N386-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: F8DF5AE74E598724	FASTA	251	28,221
	10 20 30 40 50 60 MWIGIISLFP EMFKAITDFG VTGRAVKQDL LQIQCWNPRD FTFDKHKTVD DRPYGGGPGM 70 80 90 100 110 120 LMMVQPLRDA IHAAKQAAKA EDGVEAKVIY LSPQGRKLDQ QGVKTLASNQ KLILICGRYE 130 140 150 160 170 180 GVDERLIQTE VDEEWSIGDY VLTGGELPAM TLIDAVARFV PGVLGKQASA DEDSFATGLL 190 200 210 220 230 240 DCPHYTRPEM LDGIPVPEVL MSGHHENIRK WRLEQSLERT WLRRPELLDS LALTDEQRVL 250 LAKIKKQHKI S

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74872.1.
RefSeq	YP_001054477.1.
3D structure databases
SMR	A3N386. Positions 1-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N386.
Genome annotation databases
GeneID	4848951.
GenomeReviews	Gene locus APL_1788 in contig CP000569_GR.
KEGG	apl:APL_1788.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ACTP2

Accession

Primary (citable) accession number: A3N386

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)