A3N2T5 (MTNN_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Short name=MTA/SAH nucleosidase
Short name=MTAN
EC=3.2.2.9

Alternative name(s):
5'-methylthioadenosine nucleosidase
Short name=MTA nucleosidase
S-adenosylhomocysteine nucleosidase
Short name=AdoHcy nucleosidase
Short name=SAH nucleosidase
Short name=SRH nucleosidase

Gene names

Name:	mtnN
Ordered Locus Names:	APL_1637

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: EC methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684		PRO_0000359266
Regions
	Region	175 – 176	2	Substrate binding By similarity
Sites
	Active site	14	1	Proton acceptor By similarity
	Binding site	80	1	Substrate; via amide nitrogen By similarity
	Binding site	199	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N2T5 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 627570E721E3A40C	FASTA	232	24,412
	10 20 30 40 50 60 MRLKIGIIGA MAQEVEILRN LMVEAKVIEI AGCKIYDGKI NNTQVALLQS GIGKVAAAVG 70 80 90 100 110 120 TALLLELTKP DVIINTGSAG GLDANLNVGD IVISTEVRHH DADVTAFGYE KGQLPANPAA 130 140 150 160 170 180 FLPNEQLVSV ALKETQTAGF NAVSGLICSG DVFVNGAEKI AQIRQDFPNV AAVEMEAAAI 190 200 210 220 230 AQVCHAFNVP FVVVRAISDV ADKESHLSFD EFLPLAAKNS SEIVVAMLNN FA

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN74721.1.
RefSeq	YP_001054326.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3N2T5.
SMR	A3N2T5. Positions 3-232.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N2T5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4850348.
GenomeReviews	Gene locus APL_1637 in contig CP000569_GR.
KEGG	apl:APL_1637.
PATRIC	20748307. VBIActPle94089_1703.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0775.
HOGENOM	HBG367723.
OMA	HLDNAQE.
PhylomeDB	A3N2T5.
ProtClustDB	CLSK918996.
Enzyme and pathway databases
BioCyc	APLE416269:APL_1637-MONOMER.
Family and domain databases
HAMAP	MF_01684. Salvage_tnN. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K01243.
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNN_ACTP2

Accession

Primary (citable) accession number: A3N2T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program