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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79S-adenosyl-L-methionineUniRule annotation1
Binding sitei104S-adenosyl-L-methionineUniRule annotation1
Binding sitei131S-adenosyl-L-methionineUniRule annotation1
Active sitei154By similarity1
Binding sitei154S-adenosyl-L-methionineUniRule annotation1
Binding sitei158SubstrateUniRule annotation1
Binding sitei190SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:APL_1383
OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
Taxonomic identifieri416269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001432 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000643841 – 250tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST250

Interactioni

Protein-protein interaction databases

STRINGi416269.APL_1383.

Structurei

3D structure databases

ProteinModelPortaliA3N231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni228 – 231Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3N231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKQTFADK KRKTVEQAEF TEDGRYLRKV RSFVLRTGRL SDYQRDMMNN
60 70 80 90 100
NWANLGLDYQ NTPFNFEQIF GNNNPVVLEI GFGMGRSLVE MAKQNPDRNY
110 120 130 140 150
IGIEVHTPGV GACIAYALEK GVKNLRVICH DATEILRDAI ADGSLGGLQL
160 170 180 190 200
YFPDPWQKAK HHKRRIVQPE FITRVLTKLG DNGFIHFATD WENYAEHMLE
210 220 230 240 250
VLRQFDKELR NTSATNDFIP RPDFRPLTKF EERAHRLGHG VWDLYFVKQA
Length:250
Mass (Da):29,025
Last modified:April 3, 2007 - v1
Checksum:iB0496CCD86417F5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000569 Genomic DNA. Translation: ABN74467.1.
RefSeqiWP_009875455.1. NC_009053.1.

Genome annotation databases

EnsemblBacteriaiABN74467; ABN74467; APL_1383.
GeneIDi4850187.
KEGGiapl:APL_1383.
PATRICi20747751. VBIActPle94089_1441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000569 Genomic DNA. Translation: ABN74467.1.
RefSeqiWP_009875455.1. NC_009053.1.

3D structure databases

ProteinModelPortaliA3N231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416269.APL_1383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN74467; ABN74467; APL_1383.
GeneIDi4850187.
KEGGiapl:APL_1383.
PATRICi20747751. VBIActPle94089_1441.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_ACTP2
AccessioniPrimary (citable) accession number: A3N231
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 3, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.