Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A3N231 (TRMB_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:APL_1383
OrganismActinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]
Taxonomic identifier416269 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000064384

Regions

Region228 – 2314Substrate binding By similarity

Sites

Active site1541 By similarity
Binding site791S-adenosyl-L-methionine By similarity
Binding site1041S-adenosyl-L-methionine By similarity
Binding site1311S-adenosyl-L-methionine By similarity
Binding site1541S-adenosyl-L-methionine By similarity
Binding site1581Substrate By similarity
Binding site1901Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A3N231 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: B0496CCD86417F5D

FASTA25029,025
        10         20         30         40         50         60 
MSEKQTFADK KRKTVEQAEF TEDGRYLRKV RSFVLRTGRL SDYQRDMMNN NWANLGLDYQ 

        70         80         90        100        110        120 
NTPFNFEQIF GNNNPVVLEI GFGMGRSLVE MAKQNPDRNY IGIEVHTPGV GACIAYALEK 

       130        140        150        160        170        180 
GVKNLRVICH DATEILRDAI ADGSLGGLQL YFPDPWQKAK HHKRRIVQPE FITRVLTKLG 

       190        200        210        220        230        240 
DNGFIHFATD WENYAEHMLE VLRQFDKELR NTSATNDFIP RPDFRPLTKF EERAHRLGHG 

       250 
VWDLYFVKQA 

« Hide

References

[1]"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000569 Genomic DNA. Translation: ABN74467.1.
RefSeqYP_001054072.1. NC_009053.1.

3D structure databases

ProteinModelPortalA3N231.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416269.APL_1383.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN74467; ABN74467; APL_1383.
GeneID4850187.
KEGGapl:APL_1383.
PATRIC20747751. VBIActPle94089_1441.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAFATDWEN.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycAPLE416269:GHV7-1418-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ACTP2
AccessionPrimary (citable) accession number: A3N231
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 3, 2007
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways