Reviewed, UniProtKB/Swiss-Prot A3N207 (DSBD_ACTP2)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	APL_1359

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 583	563	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_0000304382
Regions
	Transmembrane	185 – 205	21	Potential
	Transmembrane	237 – 257	21	Potential
	Transmembrane	261 – 281	21	Potential
	Transmembrane	302 – 322	21	Potential
	Transmembrane	344 – 364	21	Potential
	Transmembrane	375 – 395	21	Potential
	Transmembrane	405 – 425	21	Potential
	Transmembrane	433 – 453	21	Potential
	Domain	440 – 583	144	Thioredoxin
Amino acid modifications
	Disulfide bond	123 ↔ 128		Redox-active By similarity
	Disulfide bond	200 ↔ 322		Redox-active By similarity
	Disulfide bond	500 ↔ 503		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N207-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 46DC55D0A23EA722	FASTA	583	64,037
	10 20 30 40 50 60 MLKRFIFLLV GITLTLSAHA GLFGNDQPKY LSGAEAFAFS ATPKSDKQIE LNWQIADGYY 70 80 90 100 110 120 LYKKEIQVTP QNAEIQPLAF PAAENYHDEF FGEVEIFRNQ LTLPITFSAE QANASLSVHY 130 140 150 160 170 180 QGCTKGFCYP PETVTVSLDG QQAVDSAQNV AKNRENSTAS QPLANAPKAE QDQLAENLAN 190 200 210 220 230 240 NRLSIFWFFL LGIGLAFTPC VLPMLPLLSA IVIGNKQRPN TFKALLLSFA YVQGMALTYT 250 260 270 280 290 300 LLGLVVAAIG LPFQVALQSP PVLISLAILF TILAASMFGL FEIRLPNSWQ QKLNAMSQKQ 310 320 330 340 350 360 QGGAFGSVFV MGMIAGLVAS PCTSAPLSGA LLYVAQSGDL LTGGLALYLL ALGMGIPLIL 370 380 390 400 410 420 ITLFGNRILP KSGDWLLKVK TAFGFVMLAL PVFLLSRILP SHYEPLMWSA LAMVFVGWLI 430 440 450 460 470 480 SVIPTQGVIK QAVRIVLFLT FAVASYPWAN LVWNQGNSSH SAQVSNHLAF ERVQSLAELQ 490 500 510 520 530 540 EKLTASQGKK VMLDLYADWC VACKEFEKYT FTDQAVQQKL AEMVVLQVDM TNNSAQNDEL 550 560 570 580 MKHFNVLGLP TILFFDESGK ELTQSRVTGF LEANQFLNWL NQL

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74443.1.
RefSeq	YP_001054048.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4849875.
GenomeReviews	Gene locus APL_1359 in contig CP000569_GR.
KEGG	apl:APL_1359.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A3N207. PVFLLSR.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF02683. DsbD. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_ACTP2

Accession

Primary (citable) accession number: A3N207

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 3, 2007
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)