Reviewed, UniProtKB/Swiss-Prot A3N1V2 (DNLJ_ACTP2)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	APL_1302

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity.
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 683	683	DNA ligase HAMAP MF_01588		PRO_0000313103
Regions
	Domain	604 – 683	80	BRCT
	Nucleotide binding	43 – 47	5	NAD By similarity
	Nucleotide binding	92 – 93	2	NAD By similarity
Sites
	Active site	127	1	N6-AMP-lysine intermediate By similarity
	Metal binding	421	1	Zinc By similarity
	Metal binding	424	1	Zinc By similarity
	Metal binding	439	1	Zinc By similarity
	Metal binding	445	1	Zinc By similarity
	Binding site	125	1	NAD By similarity
	Binding site	148	1	NAD By similarity
	Binding site	185	1	NAD By similarity
	Binding site	303	1	NAD By similarity
	Binding site	327	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N1V2-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 5F849EDF05524513	FASTA	683	75,941
	10 20 30 40 50 60 MPLLSQMQEY QADTTFAQLE ALRQKLREYE YHYHVLDNPL VPDAEYDRLM NELKNLEWQH 70 80 90 100 110 120 PEWITVDSPT QRVGAKPLDG FAQVTHEIPM LSLDNAFSDE ELDGFLRRME SYITEDPHTL 130 140 150 160 170 180 AFCCEPKLDG LAVSILYVDG VLSQAATRGD GSTGEDITSN IRTIRNIPLK LNMDNPPARL 190 200 210 220 230 240 EVRGEVFMPQ KGFEELNERA LEKGEKTFAN PRNAAAGSLR QLDPKITRQR PLVLNAYGIG 250 260 270 280 290 300 VYESDDELPA THFERLQWLK SIGIPVNNEI RLATGREQLL AFYANIQAKR PTLGYDIDGT 310 320 330 340 350 360 VLKVNDIGLQ EQLGFISRSP RWAIAYKFPS QEEMTVLNDV EFQVGRTGAI TPVAKLEPVF 370 380 390 400 410 420 VAGVTVSNAT LHNGDEIERL GIVIGDTVII RRAGDVIPQI VGVVMDRRPE NAKKIEFPTA 430 440 450 460 470 480 CPVCESAVVR VEGEAVARCT GGLFCGAQRK EALKHFVSRK AMDIDGVGEK LIEQLMEREL 490 500 510 520 530 540 VHTPADLFKL DHTTLMRLER MGEKSAQNAL NSIEKAKNTT LARFLFALGI RDVGEATAQN 550 560 570 580 590 600 LANHFHNLAA IRTATFEQLQ AVQDVGEVVA NRIVRFWQEP HNVAVVEDLI SQGVHWQDVI 610 620 630 640 650 660 QVEIADNPLK GKNVVLTGTL TQLTRDQAKA LLQSFGCKVS GSVSSKTDYL IAGEKAGSKL 670 680 AKAQELGVKI LTEQEFIALT GEN

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74388.1.
RefSeq	YP_001053993.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3N1V2.
Genome annotation databases
GeneID	4849676.
GenomeReviews	Gene locus APL_1302 in contig CP000569_GR.
KEGG	apl:APL_1302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YKFPAQE.
Family and domain databases
HAMAP	MF_01588. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR000445. HhH_motif. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. PF00633. HHH. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
ProDom	PD003944. DNAligase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 4 hits. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_ACTP2

Accession

Primary (citable) accession number: A3N1V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)