Reviewed, UniProtKB/Swiss-Prot A3N1U6 (MDH_ACTP2)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	APL_1296

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Malate dehydrogenase		PRO_0000294291
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	231	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N1U6-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 0108DFF580351B35	FASTA	317	33,347
	10 20 30 40 50 60 MKVALLGAAG GIGQTLALLL KLRLPVGTDL ALYDISPVTP GIAVDISHIP TSVSAVGYSG 70 80 90 100 110 120 EDPSEALKGA NLVIITAGVA RKPGMTRADL FNINADIVKN LVEKVAEVCP KACIGIVTNP 130 140 150 160 170 180 VNTLVPIAAE VLRKAGVYDK RKLFGVTTLD VVRAKTFTSE LKEKHVETVK VPVIGGHSGP 190 200 210 220 230 240 TILPLLSQAL SEGLPLSFTQ EEIEALTYRI QNAGTEVVEA KAGGGSATLS MAESGARFAV 250 260 270 280 290 300 AVFKALLGED CVRYAYVESK EGSGYPEFFA HPVRFGLTGV EELLPIGKLS EYEQAKLGEL 310 KPVLEADIAL GKNFVNP

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74382.1.
RefSeq	YP_001053987.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4850462.
GenomeReviews	Gene locus APL_1296 in contig CP000569_GR.
KEGG	apl:APL_1296.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01516. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR010097. Malate_DHase_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_ACTP2

Accession

Primary (citable) accession number: A3N1U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)