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A3N1U6 (MDH_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:APL_1296
OrganismActinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]
Taxonomic identifier416269 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01516

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Malate dehydrogenase HAMAP-Rule MF_01516
PRO_0000294291

Regions

Nucleotide binding7 – 137NAD By similarity
Nucleotide binding117 – 1193NAD By similarity

Sites

Active site1771Proton acceptor By similarity
Binding site341NAD By similarity
Binding site811Substrate By similarity
Binding site871Substrate By similarity
Binding site941NAD By similarity
Binding site1191Substrate By similarity
Binding site1531Substrate By similarity
Binding site2311NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A3N1U6 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 0108DFF580351B35

FASTA31733,347
        10         20         30         40         50         60 
MKVALLGAAG GIGQTLALLL KLRLPVGTDL ALYDISPVTP GIAVDISHIP TSVSAVGYSG 

        70         80         90        100        110        120 
EDPSEALKGA NLVIITAGVA RKPGMTRADL FNINADIVKN LVEKVAEVCP KACIGIVTNP 

       130        140        150        160        170        180 
VNTLVPIAAE VLRKAGVYDK RKLFGVTTLD VVRAKTFTSE LKEKHVETVK VPVIGGHSGP 

       190        200        210        220        230        240 
TILPLLSQAL SEGLPLSFTQ EEIEALTYRI QNAGTEVVEA KAGGGSATLS MAESGARFAV 

       250        260        270        280        290        300 
AVFKALLGED CVRYAYVESK EGSGYPEFFA HPVRFGLTGV EELLPIGKLS EYEQAKLGEL 

       310 
KPVLEADIAL GKNFVNP 

« Hide

References

[1]"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000569 Genomic DNA. Translation: ABN74382.1.
RefSeqYP_001053987.1. NC_009053.1.

3D structure databases

ProteinModelPortalA3N1U6.
SMRA3N1U6. Positions 1-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416269.APL_1296.

Proteomic databases

PRIDEA3N1U6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN74382; ABN74382; APL_1296.
GeneID4850462.
KEGGapl:APL_1296.
PATRIC20747547. VBIActPle94089_1353.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213792.
KOK00024.
OMAVEVKGFA.
OrthoDBEOG6091FG.
ProtClustDBPRK05086.

Enzyme and pathway databases

BioCycAPLE416269:GHV7-1318-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01516. Malate_dehydrog_1.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PTHR11540:SF1. PTHR11540:SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_ACTP2
AccessionPrimary (citable) accession number: A3N1U6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families