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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei251ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAPLE416269:G1G87-1339-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:APL_1275
OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
Taxonomic identifieri416269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001432 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000018661 – 479Glutamate--tRNA ligaseAdd BLAST479

Proteomic databases

PRIDEiA3N1S5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi416269.APL_1275

Structurei

3D structure databases

ProteinModelPortaliA3N1S5
SMRiA3N1S5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 31"HIGH" regionUniRule annotationAdd BLAST11
Motifi248 – 252"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiAYYAFDT

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A3N1S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIETLFPLD PNVKVRTRFA PSPTGYLHVG GARTALSSWL YAKHFNGEFV
60 70 80 90 100
LRIEDTDLER STPEATAAIL EGMEWLNLAW EHGPYYQTKR FDRYNQVIDQ
110 120 130 140 150
MIEQGLAYRC YCSKERLENL RHEQEANKEK PRYDRHCLAH HDQPTDAPHV
160 170 180 190 200
VRFKNPQEGS VVFDDAVRGR IEISNSELDD LIIRRTDGSP TYNFCVVVDD
210 220 230 240 250
WDMGITHVVR GEDHINNTPR QINILKALGA PIPTYAHVSM INGDDGQKLS
260 270 280 290 300
KRHGAVSVMQ YRDDGYLPEA LINYLVRLGW GHGDQEIFSR EEMIELFDIH
310 320 330 340 350
SVSKSASAFN TDKLQWLNQH YMRSLPAEHV AKYLAWHMND QAIDTSSGPA
360 370 380 390 400
LEEIIPVLSE RAKTLKELAA ASRYFYQEFD GYDEKAAAKN FKAEAVAPLA
410 420 430 440 450
KLLEKLTALT DWSVEAIHDA MNATAADLEI GMGKVGMPFR LAVTGSGQSP
460 470
SMDITAKLVG RERTLARIQK AIEFIQAQA
Length:479
Mass (Da):54,190
Last modified:April 3, 2007 - v1
Checksum:i4773629B891ACF97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000569 Genomic DNA Translation: ABN74361.1
RefSeqiWP_011848558.1, NC_009053.1

Genome annotation databases

EnsemblBacteriaiABN74361; ABN74361; APL_1275
GeneIDi4849358
KEGGiapl:APL_1275
PATRICifig|416269.6.peg.1331

Similar proteinsi

Entry informationi

Entry nameiSYE_ACTP2
AccessioniPrimary (citable) accession number: A3N1S5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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