Reviewed, UniProtKB/Swiss-Prot A3N1Q1 (PGK_ACTP2)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	APL_1251

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Phosphoglycerate kinase HAMAP MF_00145		PRO_1000057952
Regions
	Nucleotide binding	344 – 347	4	ATP By similarity
	Region	21 – 23	3	Substrate binding By similarity
	Region	59 – 62	4	Substrate binding By similarity
Sites
	Binding site	36	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	198	1	ATP By similarity
	Binding site	315	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N1Q1-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 88042F440E009A86	FASTA	391	41,170
	10 20 30 40 50 60 MSVIKMADLD LAGKRLFIRA DLNVPVKDGK VTSDARIRAT IPTLKLALQK GAKVMVTSHL 70 80 90 100 110 120 GRPTEGVFEE ANSLQPVVDY LNASDLGVPV RLVRDYLDGV EVAENEIVVL ENVRINKGEK 130 140 150 160 170 180 KNDPELAKKY AALCDVFVMD AFGTAHRAEG STYGVAEYAP VACAGPLLAA ELDALGKALK 190 200 210 220 230 240 EPQRPMLAIV GGSKVSTKLT VLDSLSKIAD QLIVGGGIAN TFIAAEGHPV GKSLYEADLI 250 260 270 280 290 300 PEAKRLAAAT NIPVPVDVRV GTEFSEIAPA TEKAVSEVQA DESIFDIGDK SAEELANIIK 310 320 330 340 350 360 SAKTILWNGP VGVFEFPNFR KGTEVISNAI AEATANGAFS IAGGGDTLAA IDLFGIADKI 370 380 390 SYISTGGGAF LEFVEGKVLP AVEILEKRAN G

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74337.1.
RefSeq	YP_001053942.1.
3D structure databases
SMR	A3N1Q1. Positions 2-389.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N1Q1.
Genome annotation databases
GeneID	4849213.
GenomeReviews	Gene locus APL_1251 in contig CP000569_GR.
KEGG	apl:APL_1251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CLENTRF.
Family and domain databases
HAMAP	MF_00145. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_ACTP2

Accession

Primary (citable) accession number: A3N1Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)