A3N1F9 (METE_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	APL_1155

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017215
Sites
	Metal binding	642	1	Zinc By similarity
	Metal binding	644	1	Zinc By similarity
	Metal binding	727	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N1F9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 9BA041FEC80DE04E	FASTA	757	85,886
	10 20 30 40 50 60 MTTLHILGFP RVGAKRELKF AQERYWRKEL AEQDLLDLAK ALREKNWLHQ AQADADFVTV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSQTL SLDQYFQLAR GNKEQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFQKNTT FKANPTHYVN QIREAKAAGH QVKPVIVGPL TFLWLGKEKG EAFNRFDLLK 190 200 210 220 230 240 QLVPIYFEIL TALAAEGVEW IQIDEPALAL DLPQEWLAAY QDVYAQLAKV NAKLLLATYF 250 260 270 280 290 300 GSVAEHADLL KALPVDGLHL DLVRAPEQLS AFEDYPKVLS AGVIDGRNIW RANLNRVLDV 310 320 330 340 350 360 LEPLKAKFNQ RLWIAPSCSL LHTPYDLAVE TQLQQNNPEL YRWLAFTLQK VAELQVLKQA 370 380 390 400 410 420 LNAGRDAVTE QLNDSQTAAD ARANSNVIHK AEVAQRLANL PANADKRQSP FAERIKLQNK 430 440 450 460 470 480 WLNLPLLPTT NIGSFPQTLE IRHARAAFKK GELSLADYEA AMKKEIEFVV RKQEELDLDV 490 500 510 520 530 540 LVHGEGERND MVEYFGELLD GFAFTKFGWV QSYGSRCVKP PVIYGDVTRP EPMTVRWSQY 550 560 570 580 590 600 AQSLTDKVMK GMLTGPVTIL QWSFVRNDIP RSTVCKQIGV ALSDEVLDLE KAGIKVIQID 610 620 630 640 650 660 EPAIREGLPL KRADWDAYLQ WAGEAFRLSY MGVKDDTQIH THMCYSEFND ILPAIAGLDA 670 680 690 700 710 720 DVITIETSRS DMELLTAFAD FKYPNDIGPG VYDIHSPRVP TAGEIEHLLR KALKVIPKER 730 740 750 LWVNPDCGLK TRGWKETIEQ LQVMVEVTKK LRAELAE

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN74245.1.
RefSeq	YP_001053850.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3N1F9.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N1F9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4849095.
GenomeReviews	Gene locus APL_1155 in contig CP000569_GR.
KEGG	apl:APL_1155.
PATRIC	20747234. VBIActPle94089_1204.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	A3N1F9.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	APLE416269:APL_1155-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_ACTP2

Accession

Primary (citable) accession number: A3N1F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program