Reviewed, UniProtKB/Swiss-Prot A3N1F3 (SYD_ACTP2)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	APL_1149

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000006628

Sequences

	Sequence		Length	Mass (Da)
	A3N1F3-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 3CFE6D25A9076D8B	FASTA	591	66,809
	10 20 30 40 50 60 MMRSHYCGAL NRSHVGQTVT LSGWVHRVRN LGRFIFMQIR DREGIVQVFF DEKDEAIFKI 70 80 90 100 110 120 ASSLRSEACV QIQGEVIARD ESQINKEMAT GEIEVLVKNV VVYNNADVLP LDFNQNNTEE 130 140 150 160 170 180 QRLKYRYLDL RRPEMAEKLK TRAKITSFVR RYMDDNGFLD IETPMLTKAT PEGARDYLVP 190 200 210 220 230 240 SRVHNGKFYA LPQSPQLFKQ LLMMSGFDRY YQIVKCFRDE DLRADRQPEF TQIDVETSFL 250 260 270 280 290 300 TAEEVRELME NMIHGLWLDR LNVDLGKFPI MTWQEAMQRF GSDKPDLRNP LELVDVADIL 310 320 330 340 350 360 KDVEFKVFNE PANSADGRVT VLRVPNGASL TRKQIDEYTQ FVGIYGAKGL AWAKINNVNA 370 380 390 400 410 420 GMEGIQSPVA KFLNEEVFKA LIERTNATSG DILFFGADKW QVVTDSMGAL RLKVGRDLAL 430 440 450 460 470 480 TDLSAWKPLW VIDFPMFEKD DEGNLSAMHH PFTSPKNLTP EELAANPVNA VANAYDMVIN 490 500 510 520 530 540 GYEVGGGSVR IYDPKMQQTV FGILGINEQD QQEKFGFLLD ALKFGTPPHA GLAFGLDRLT 550 560 570 580 590 MLITGTENIR DVIAFPKTTA AACLMTEAPS FANPQALEEL GIAVLKKEKA E

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74239.1.
RefSeq	YP_001053844.1.
3D structure databases
SMR	A3N1F3. Positions 2-586.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N1F3.
Genome annotation databases
GeneID	4849850.
GenomeReviews	Gene locus APL_1149 in contig CP000569_GR.
KEGG	apl:APL_1149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VDRRRDH.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACTP2

Accession

Primary (citable) accession number: A3N1F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)