Reviewed, UniProtKB/Swiss-Prot A3N1C8 (K6PF_ACTP2)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	APL_1124

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	6-phosphofructokinase HAMAP MF_00339		PRO_1000059734
Regions
	Nucleotide binding	25 – 29	5	ATP By similarity
	Nucleotide binding	159 – 163	5	ATP By similarity
	Nucleotide binding	176 – 192	17	ATP By similarity
Sites
	Active site	132	1	Proton acceptor By similarity
	Metal binding	190	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	192	1	Magnesium By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Binding site	257	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N1C8-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 1D2C62562BBD6586	FASTA	324	35,515
	10 20 30 40 50 60 MAKQIKKIAV LTSGGDAPGM NAAIRGVVRA ALNEGLEVYG VQDGYYGLYT DRVIPLDRRS 70 80 90 100 110 120 VSETINRGGT FLGSARFPQF KDPDVRKKSV ETLKKYDIDA LVVIGGDGSY MGAKLITEEF 130 140 150 160 170 180 GYPCIGIPGT IDNDIVGTDY TIGYQTALET AVEAIDRLRD TSTSHQRISI VEIMGRHCGD 190 200 210 220 230 240 LTISAALASG CEYIIVPEKG LDKESLMRNI EDGFNKGKRH AIIAITELMT DVQALAKEIE 250 260 270 280 290 300 DRFGHETRAT VLGHIQRGGA PCPFDRILAS RMGVYAVDLL LQGHGGRCIG IKNENLVHHD 310 320 IIDAINNMRR PFKEELFEAA RKLF

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN74214.1.
RefSeq	YP_001053819.1.
3D structure databases
SMR	A3N1C8. Positions 5-324.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N1C8.
Genome annotation databases
GeneID	4849861.
GenomeReviews	Gene locus APL_1124 in contig CP000569_GR.
KEGG	apl:APL_1124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DTCLNTV.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_ACTP2

Accession

Primary (citable) accession number: A3N1C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)