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A3N1C8

- PFKA_ACTP2

UniProt

A3N1C8 - PFKA_ACTP2

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
    Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
    Active sitei132 – 1321Proton acceptorUniRule annotation
    Binding sitei159 – 1591Allosteric activator ADPUniRule annotation
    Binding sitei167 – 1671Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei216 – 2161Allosteric activator ADPUniRule annotation
    Binding sitei227 – 2271SubstrateUniRule annotation
    Binding sitei248 – 2481Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 772ATPUniRule annotation
    Nucleotide bindingi106 – 1094ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAPLE416269:GHV7-1136-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:APL_1124
    OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
    Taxonomic identifieri416269 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
    ProteomesiUP000001432: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324ATP-dependent 6-phosphofructokinasePRO_1000059734Add
    BLAST

    Proteomic databases

    PRIDEiA3N1C8.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi416269.APL_1124.

    Structurei

    3D structure databases

    ProteinModelPortaliA3N1C8.
    SMRiA3N1C8. Positions 5-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni130 – 1323Substrate bindingUniRule annotation
    Regioni174 – 1763Substrate bindingUniRule annotation
    Regioni190 – 1923Allosteric activator ADP bindingUniRule annotation
    Regioni218 – 2203Allosteric activator ADP bindingUniRule annotation
    Regioni254 – 2574Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiGFGGRCV.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3N1C8-1 [UniParc]FASTAAdd to Basket

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    MAKQIKKIAV LTSGGDAPGM NAAIRGVVRA ALNEGLEVYG VQDGYYGLYT    50
    DRVIPLDRRS VSETINRGGT FLGSARFPQF KDPDVRKKSV ETLKKYDIDA 100
    LVVIGGDGSY MGAKLITEEF GYPCIGIPGT IDNDIVGTDY TIGYQTALET 150
    AVEAIDRLRD TSTSHQRISI VEIMGRHCGD LTISAALASG CEYIIVPEKG 200
    LDKESLMRNI EDGFNKGKRH AIIAITELMT DVQALAKEIE DRFGHETRAT 250
    VLGHIQRGGA PCPFDRILAS RMGVYAVDLL LQGHGGRCIG IKNENLVHHD 300
    IIDAINNMRR PFKEELFEAA RKLF 324
    Length:324
    Mass (Da):35,515
    Last modified:April 3, 2007 - v1
    Checksum:i1D2C62562BBD6586
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN74214.1.
    RefSeqiYP_001053819.1. NC_009053.1.

    Genome annotation databases

    EnsemblBacteriaiABN74214; ABN74214; APL_1124.
    GeneIDi4849861.
    KEGGiapl:APL_1124.
    PATRICi20747160. VBIActPle94089_1172.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN74214.1 .
    RefSeqi YP_001053819.1. NC_009053.1.

    3D structure databases

    ProteinModelPortali A3N1C8.
    SMRi A3N1C8. Positions 5-324.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416269.APL_1124.

    Proteomic databases

    PRIDEi A3N1C8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN74214 ; ABN74214 ; APL_1124 .
    GeneIDi 4849861.
    KEGGi apl:APL_1124.
    PATRICi 20747160. VBIActPle94089_1172.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi GFGGRCV.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci APLE416269:GHV7-1136-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
      Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
      J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: L20.

    Entry informationi

    Entry nameiPFKA_ACTP2
    AccessioniPrimary (citable) accession number: A3N1C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3