Reviewed, UniProtKB/Swiss-Prot A3N1C4 (GCP_ACTP2)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	APL_1120

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303246
Sites
	Metal binding	111	1	Zinc Potential
	Metal binding	115	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A3N1C4-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: E18B7948F4819501	FASTA	347	37,518
	10 20 30 40 50 60 MRILGIETSC DETGVAIYDE EKGLVANQLY SQIEMHADYG GVVPELASRD HIRKTLPLIQ 70 80 90 100 110 120 EALKEANLTA ADIDGVVYTA GPGLVGALLV GSTIARSLAY AWNVPALGVH HMEGHLMAPM 130 140 150 160 170 180 LEDNPPAFPF VALLISGGHT QLVKVEGVGQ YEILGESIDD AAGEAFDKTG KLLGLDYPAG 190 200 210 220 230 240 VAVSQLAEKG TPNRFVFPRP MTDRPGLDFS FSGLKTFAAN TINANLDENG RLDEQTRCDI 250 260 270 280 290 300 AHAFQQAVVD TIIIKCKRAL QQTGYKRLVM AGGVSANKQL RTDLAEMMKN LKGEVYYPRP 310 320 330 340 QFCTDNGAMI AYTGFLRLKN GETSDLSISV KPRWNMTELP DISTTGG

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN74210.1.
RefSeq	YP_001053815.1.
3D structure databases
SMR	A3N1C4. Positions 1-341.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N1C4.
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	4848639.
GenomeReviews	Gene locus APL_1120 in contig CP000569_GR.
KEGG	apl:APL_1120.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0533.
HOGENOM	HBG304663.
OMA	CKRALKQ.
PhylomeDB	A3N1C4.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_ACTP2

Accession

Primary (citable) accession number: A3N1C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)