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A3N1B7 (NAGZ_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:APL_1111
OrganismActinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]
Taxonomic identifier416269 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000005649

Regions

Region162 – 1632Substrate binding By similarity

Sites

Active site1751Proton donor/acceptor By similarity
Active site2471Nucleophile By similarity
Binding site601Substrate By similarity
Binding site681Substrate By similarity
Binding site1321Substrate By similarity
Site1731Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A3N1B7 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: AB16DBBA7B4F3CDD

FASTA34538,994
        10         20         30         40         50         60 
MLLIDIKDKE LSQEEVEILE HPLVSGLILF SRNFHDKVQL EALVKSIRQR VKKPLLITVD 

        70         80         90        100        110        120 
QEGGRVQRFR EGFTKLPAMQ AFHTLAKNPQ ESTALARQTG WLMAAEMFAL DIDLSFAPVL 

       130        140        150        160        170        180 
DLGHQCKAIG DRSFGENPDA MLPIAEAFID GMREMGMATT GKHFPGHGHV LADSHLETPF 

       190        200        210        220        230        240 
DDRPKEAIFN RDILPFKQLI SKGKLSAIMP AHVIYTQCDS QPASGSEYWL KQVLRNQLNF 

       250        260        270        280        290        300 
NGVIFSDDLG MKGAGFMGNF VERSEKAIHA GCDLLLLCNE PEGVIQVLDG LKYQPTKTQT 

       310        320        330        340 
ERHISLMKRK TVRWNELEAS PRYQQAQQRL TALQNDWLEY KAQHC 

« Hide

References

[1]"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000569 Genomic DNA. Translation: ABN74203.1.
RefSeqYP_001053808.1. NC_009053.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416269.APL_1111.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN74203; ABN74203; APL_1111.
GeneID4849180.
KEGGapl:APL_1111.
PATRIC20747134. VBIActPle94089_1159.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAHILALYE.
OrthoDBEOG6BCT06.
ProtClustDBPRK05337.

Enzyme and pathway databases

BioCycAPLE416269:GHV7-1124-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_ACTP2
AccessionPrimary (citable) accession number: A3N1B7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries